二氧化硅纳米材料SSN-2的递送能力与安全性评价  

Evaluation of Delivery performance and biosafety of silica nanomaterial SSN-2

作　　者：魏波 薛洁 张金 王力 王鑫 WEI Bo;XUE Jie;ZHANG Jin;WANG Li;WANG Xin(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Center for Innovation Marine Drug Screening&Evaluation,Laboratory for Marine Drugs and Bioproducts,Qingdao Marine Science and Technology Center,Qingdao 266237,China;Fang Zongxi Center for Marine Evo-Devo,MoE Key Laboratory of Marine Genetics and Breeding,School of Marine Life Sciences,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]青岛海洋科技中心,海洋创新药物筛选与评价平台,海洋药物与生物制品功能实验室,山东青岛266237 [3]中国海洋大学方宗熙海洋生物进化与发育研究中心,海洋生物遗传学与育种教育部重点实验室,海洋生命学院,山东青岛266003

出　　处：《中国海洋药物》2025年第1期71-77,共7页Chinese Journal of Marine Drugs

基　　金：山东省自然科学基金项目(ZR202110150015,ZR2021LSW013,ZR202110290057)资助。

摘　　要：目的对新型药物和基因递送载体SSN-2在海洋药物领域的应用和安全性进行评价。方法通过细胞转染、荧光拍照、MTT法和溶血实验评估SSN-2的递送效果与安全性。结果SSN-2可以吸附和递送外源小干扰RNA(siRNA)至哺乳动物细胞、原代细胞和萨氏海鞘成体卵细胞,是一种优秀的药物和基因递送工具。SSN-2无明显的细胞毒性和生理溶血性。结论SSN-2是一个安全有效的遗传工具,为海洋生物和海洋药物研究提供了新工具。Objective To evaluate the application and safety of new drug and gene delivery vector SSN-2 in the field of Marine medicine.Methods The delivery efficiency and safety of SSN-2 were evaluated by cell transfection,fluorescence photography,MTT assay and hemolysis analysis.Results SSN-2 could adsorb and delivery the exogenous siRNA to Mammalian cells,primary cells,and fertilized eggs of Ciona savignyi as an excellent delivery vehicle for drugs and genes.SSN-2 has no obvious cytotoxicity and physiological hemolysis.Conclusion SSN-2 is a safe and effective genetic tool that provides a new tool for the study of Marine organisms and Marine drugs.

关 键 词：二氧化硅纳米材料 递送siRNA 遗传工具 安全性 

分 类 号：R931.77[医药卫生—生药学]