Regulation of mitochondrial network architecture and function in mesenchymal stem cells by micropatterned surfaces  

作　　者：Zixuan Dong Weiju Han Panyu Jiang Lijing Hao Xiaoling Fu 

机构地区：[1]The Second Affiliated Hospital,School of Biomedical Sciences and Engineering,South China University of Technology,Guangzhou 511442,China [2]National Engineering Research Center for Tissue Restoration and Reconstruction and Innovation Center for Tissue Restoration and Reconstruction,Guangzhou 510006,China [3]Laboratory of Biomedical Engineering of Guangdong Province,South China University of Technology,Guangzhou 510006,China [4]School of Materials Science and Engineering,South China University of Technology,Guangzhou 510006,China

出　　处：《Regenerative Biomaterials》2025年第1期47-57,共11页再生生物材料(英文版)

基　　金：supported by the National Natural Science Foundation of China(T2288101,31971266,82272152);Guangdong Basic and Applied Basic Research Foundation(2022A1515011925);National Key R&D Program of China(2021YFC2400700);Science and Technology Innovation Program of Guangdong Province Medical Products Administration(2022ZDZ11);Applied Basic Research Program of Guangzhou city of China(202201010040).

摘　　要：Mitochondrial network architecture,which is closely related to mitochondrial function,is mechanically sensitive and regulated by multiple stimuli.However,the effects of microtopographic cues on mitochondria remain poorly defined.Herein,polycaprolactone(PCL)surfaces were used as models to investigate how micropatterns regulate mitochondrial network architecture and function in rat adipose-derived stem cells(rASCs).It was found that large pit(LP)-induced rASCs to form larger and more complex mitochondrial networks.Consistently,the expression of key genes related to mitochondrial dynamics revealed that mitochondrial fusion(MFN1 and MFN2)and midzone fission(DRP1 and MFF)were increased in rASCs on LP.In contrast,the middle pit(MP)-enhanced mitochondrial biogenesis,as evidenced by the larger mitochondrial area and higher expression of PGC-1.Both LP and MP promoted ATP production in rASCs.It is likely that LP increased ATP levels through modulating mitochondrial network architecture while MP stimulated mitochondria biogenesis to do so.Our study clarified the regulation of micropatterned surfaces on mitochondria,highlighting the potential of LP and MP as a simple platform to stimulate mitochondria and the subsequent cellular function of MSCs.

关 键 词：micropatterned surface mesenchymal stem cells mitochondrial network architecture mitochondrial function regulation of mitochondria 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]