石蜡/松香混合脱毛法、硫化钠脱毛法、脱毛膏脱毛法建立的小鼠脱发模型特点对比观察  

Comparison of characteristics of mouse alopecia models established by paraffin/rosin mixed depilation method,sodium sulfide depilation method,and depilatory cream depilation method

作　　者：管堂飞 洪灿辉 杨鑫 吴广俊 肖培云 张成桂 何正春 GUAN Tangfei;HONG Canhui;YANG Xin;WU Guangjun;XIAO Peiyun;ZHANG Chenggui;HE Zhengchun(Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali University,Dali 671000,China;不详)

机构地区：[1]大理大学云南省昆虫生物医药研发重点实验室,云南大理671000 [2]大理大学药学院,云南大理671000 [3]四川大学华西公共卫生学院/华西第四医院,四川成都610041 [4]大理大学药用特种昆虫开发国家地方联合工程研究中心,云南大理671000

出　　处：《山东医药》2025年第3期51-57,共7页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(82060747)。

摘　　要：目的对比观察采用石蜡/松香混合脱毛法、硫化钠脱毛法和脱毛膏脱毛法建立的C57BL/6J小鼠脱发模型的特点,以期为小鼠脱发模型选择提供理论依据和技术参考。方法44只SPF级雌性C57BL/6J小鼠分成4组,即A、B、C和D组,每组11只。A组正常饲养,不做脱毛处理,作为正常对照。B组采用石蜡/松香混合脱毛法建立小鼠脱发模型,C组采用硫化钠脱毛法建立小鼠脱发模型,D组采用脱毛膏脱毛法建立小鼠脱发模型,脱毛面积约2.5 cm×4.0 cm。以小鼠脱发模型制备成功为第0天,观察模型制备成功后各组小鼠的毛发生长状态,对小鼠皮肤颜色和毛发再生情况进行评分。在第15、21天时测量小鼠脱毛区域毛发长度,在第21天时测量小鼠脱毛区域毛发重量。在第21天时,采用全自动血细胞分析仪检测眼球血中白细胞(WBC)、中性粒细胞(Gran)、淋巴细胞(Lym)、单核细胞(Mon)数量,判断小鼠体内炎症情况。在第1、21天时,采用HE染色法观察小鼠皮肤组织病理变化及皮肤损伤程度,包括毳毛囊、终毛囊、总毛囊、终毛囊/毳毛囊。结果3种方法均成功建立小鼠脱发模型。脱毛后,B、C、D组小鼠脱毛区域毛发均缓慢生长,B组毛发生长状态更好,皮肤颜色评分和毛发再生情况评分更高。第15、21天时C、D组小鼠脱毛区域毛发长度均低于B组(P均<0.05)。A组小鼠脱毛区域毛发重量均高于其余各组,B组小鼠脱毛区域毛发重量均高于C、D组(P均<0.05)。与A组小鼠相比,B组Gran降低、Mon升高,C组WBC、Lym、Mon均升高,D组Mon升高;与B组小鼠相比,C组WBC、Gran、Lym、Mon均升高;与C组小鼠相比,D组WBC、Gran、Lym、Mon均降低(P均<0.05)。第21天时,B组毛囊数量增加,终毛数量增多;C组皮肤毛囊数量较少;D组可见少量终毛和毳毛,毛囊结构较完整。与A组小鼠相比,C组终毛囊数量、总毛囊数量及终毛囊/毳毛囊比均降低,D组毳毛囊数量、终毛囊数量、�Objective To comparatively observe the characteristics of alopecia models established in C57BL/6J mice using the paraffin/rosin mixed depilation method,sodium sulfide depilation method,and depilatory cream depilation method,aiming to provide a theoretical basis and technical reference for the selection of mouse alopecia models.Methods Forty-four SPF-grade female C57BL/6J mice were divided into four groups,namely groups A,B,C,and D,with 11 mice in each group.Mice in the group A were normally raised without depilation treatment and served as the normal control.In the group B,the mouse alopecia model was established using the paraffin/rosin mixed depilation method.In the group C,the model was established using the sodium sulfide depilation method.In the group D,the model was established using the depilatory cream depilation method.The depilation area was approximately 2.5 cm×4.0 cm.Taking the successful preparation of the mouse alopecia model as day 0,the hair growth status of mice in each group after the successful model preparation was observed,and the skin color and hair regeneration of mice were scored.On day 15 and 21,the hair length in the depilated area of mice was measured,and on day 21,the hair weight in the depilated area of mice was measured.On day 21,a fully automated blood cell analyzer was used to detect the numbers of white blood cells(WBC),granulocytes(Gran),lymphocytes(Lym),and monocytes(Mon)in the eyeball blood to determine the inflammatory situation in mice.On day 1 and 21,HE staining was used to observe the pathological changes and skin damage degree of mouse skin tissues,including vellus hair follicles,terminal hair follicles,total hair follicles,and the ratio of terminal hair follicles to vellus hair follicles.Results Alopecia models were successfully established in mice by all three methods.After depilation,the hair in the depilated area of mice in the groups B,C,and D grew slowly,and the hair growth status in the group B was better,with higher scores for skin color and hair regeneration.On d

关 键 词：脱发 动物模型 石蜡/松香 硫化钠 脱毛膏 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]