初级运动皮质介导电针镇痛和抗焦虑的作用研究  

Study on the involvement of primary motor cortex in the electroacupunctureinduced analgesic and anxiolytic effects

作　　者：纪若馨 唐扬 田志成 丁慧 杨新江 李祥辰 王菲 罗层 JI Ruoxin;TANG Yang;TIAN Zhicheng;DING Hui;YANG Xinjiang;LI Xiangchen;WANG Fei;LUO Ceng(School of Acupuncture and Massage,Shaanxi University of Chinese Medicine,Xianyang 712000;Department of Neurobiology,School of Basic Medicine,Air Force Medical University,Xi’an 710032;Battalion 3,No.1 Cadet Regiment,School of Basic Medical Sciences,Air Force Medical University,Xi’an 710032;Neurosurgery Department,the First Affiliated Hospital of Air Force Medical University,Xi’an 710032;Department of Rehabilitation and Physical Therapy,Xijing Hospital,Xi’an 710032;Medical Experimental Center,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学针灸推拿学院,咸阳712000 [2]空军军医大学基础医学院神经生物学教研室,西安710032 [3]空军军医大学基础医学院一大队三队,西安710032 [4]空军军医大学第一附属医院神经外科,西安710032 [5]西京医院康复理疗科,西安710032 [6]陕西中医药大学医学科研实验中心,咸阳712046

出　　处：《神经解剖学杂志》2025年第1期1-8,共8页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(32071002,82201370);博士后面上项目(2021MD703955)。

摘　　要：目的:观察电针刺激对慢性疼痛及相关负性情绪的治疗作用,探讨初级运动皮质(M1)区谷氨酸能神经元在电针(EA)刺激后的活动改变。方法:将雄性C57BL/6J小鼠随机分为4组,分别为假手术组(Sham)、单纯电针刺激组(EA)、神经损伤组(SNI)、电针治疗神经损伤组(SNI+EA)。在构建坐骨神经分支选择性损伤(SNI)模型第30 d后,在小鼠双侧足三里(ST36)给予EA刺激。采用von Frey纤维丝与Hargreaves热敏测试箱对小鼠进行机械痛敏和热痛敏的检测。采用高架十字迷宫实验(EPM)检测小鼠的焦虑样行为的影响。通过免疫荧光双标染色技术观察M1区谷氨酸能神经元c-Fos的表达。结果:与Sham组相比,SNI组小鼠在造模30 d后双侧后足产生显著的机械痛敏和热痛敏(P<0.01),并伴随明显的焦虑样行为(P<0.01)。SNI+EA组小鼠疼痛及焦虑样行为均得到显著缓解(P<0.01);SNI组小鼠M1区谷氨酸能神经元c-Fos表达显著降低;给予EA治疗后,与SNI组相比,SNI+EA组小鼠M1区谷氨酸能神经元c-Fos表达显著增多(P<0.01)。结论:EA治疗显著缓解了SNI诱致的慢性疼痛及相关焦虑样情绪,M1区谷氨酸能神经元可能在其中扮演重要角色。Objective:To observe the therapeutic effects of electroacupuncture(EA)stimulation on chronic pain and associated negative emotions,and to investigate the activity changes of glutamatergic neurons in the primary motor cortex(M1)following EA stimulation.Methods:Male C57BL/6J mice were randomly divided into four groups:sham surgery(Sham),pure electroacupuncture stimulation(EA),nerve injury(SNI)and electroacupuncture treatment of nerve injury(SNI+EA).Thirty days after the SNI model establishment,EA stimulation was administered bilaterally to the Zusanli(ST36)acupoints in mice.von Frey filaments and Hargreaves heat sensitivity testing were used to detect mechanical and thermal hyperalgesia.The elevated plus maze test was conducted to assess the impact on anxiety-like behaviors in mice.Dual immunofluorescence staining was employed to observe changes in c-Fos expression in M1 glutamatergic neurons.Results:As compared to Sham group,SNI-treated mice exhibited significant mechanical and thermal hyperalgesia in bilateral hindpaws at 30 days post-modeling(P<0.01)and displayed obvious anxiety-like behaviors(P<0.01).The SNI+EA group showed significant relief in pain and anxiety-like behaviors(P<0.01).c-Fos expression in M1 glutamatergic neurons was significantly decreased in SNI mice.Conversely,after electroacupuncture(EA)treatment,compared to the SNI group,M1 glutamatergic neurons in the SNI+EA group showed significantly increased c-Fos expression(P<0.01).Conclusion:Electroacupuncture significantly alleviated chronic pain and associated anxiety-like behaviors induced by SNI,which might involve the activation of glutamatergic neurons in the M1 in this process.

关 键 词：电针 慢性痛 初级运动皮质 谷氨酸能神经元 焦虑 小鼠 

分 类 号：R245.97[医药卫生—针灸推拿学]