跑台运动对慢性应激大鼠海马sigma-1受体和内质网应激通路的影响  

Effects of treadmill running on hippocampal sigma-1 receptor and endoplasmic reticulum stress pathway in chronically stressed rats

作　　者：傅程龙 徐迅[1] 郭燕兰 周琛婓 郭杨博 崔建梅 FU Chenglong;XU Xun;GUO Yanlan;ZHOU Chenfei;GUO Yangbo;CUI Jianmei(School of Social Development&Public Education,Huzhou Vocational&Technical College,Huzhou,313000;Physical Education Institute of Shanxi University,Taiyuan 030006;Physical Education Institute,North University of China,Taiyuan 030051,China)

机构地区：[1]湖州职业技术学院社会发展与公共教育学院,湖州313000 [2]山西大学体育学院,太原030006 [3]中北大学体育学院,太原030051

出　　处：《神经解剖学杂志》2025年第1期39-44,共6页Chinese Journal of Neuroanatomy

基　　金：山西省自然科学基金(20210302123060);2024年度山西省研究生教育创新计划(2024SJ283);山西省哲学社会科学规划课题(2023YY170)。

摘　　要：目的:通过检测海马神经元病理变化及sigma-1受体(SIR)和内质网应激(ERS)通路分子的表达,探讨跑台运动改善慢性不可预知应激(CUMS)大鼠认知障碍的机制。方法:30只SD大鼠随机分为对照组、CUMS组及CUMS运动组(CUMS+TR),每组10只大鼠。随后,CUMS组及CUMS+TR组大鼠建立CUMS抑郁模型,同时,CUMS+TR组大鼠进行4周中等强度跑台运动(20 m/min)。运动及应激结束后采用八臂迷宫(RAM)实验评估大鼠的学习记忆能力;HE染色观察大鼠海马神经元病理变化,Western blot检测海马葡萄糖调节蛋白78(GRP78)、蛋白肌醇必需酶l(IRE1)、X-盒结合蛋白1(XBP1)、环磷酸腺苷反应元件结合转录因子同源蛋白(CHOP)、半胱氨酸天冬氨酸蛋白酶(caspase-3)及SIR的表达。结果:与对照组比较,RAM实验中CUMS组大鼠总记忆错误次数增多,完成八臂迷宫时间延长(P<0.05);并且CUMS组大鼠海马神经元形态及结构呈明显病理改变、海马SIR蛋白表达减少,GRP78、IRE1、XBP1、CHOP及caspase-3蛋白表达明显增加(P<0.05)。4周跑台运动可显著改善CUMS大鼠的学习记忆能力和海马神经元的病理改变,与CUMS组比较,CUMS+TR组大鼠海马SIR表达增加,GRP78、IRE1、XBP1、CHOP及caspase-3蛋白表达明显下降(P<0.05)。结论:跑台运动可改善CUMS大鼠认知障碍,其机制可能与上调海马SIR表达及抑制ERS介导细胞凋亡通路有关。Objective:To investigate the possible mechanism of treadmill exercise(TR)in improving cognitive function of rats with chronic unpredictable mild stress(CUMS)by detecting pathological changes and the expression of sigma-1 receptor(SIR),endoplasmic reticulum stress-related proteins in hippocampus.Methods:30 SD rats were randomly allocated into control group(n=10),CUMS group(n=10)and CUMS exercise group(CUMS+TR,n=10).Subsequently,depressive model was established in rats of CUMS group and CUMS+TR group,meanwhile,CUMS+TR group rats were subjected to 4 weeks of moderate intensity treadmill exercise(20 m/min).After the CUMS and treadmill exercise,the eight-arm radial maze test(RAM)was carried out to evaluate the learning-memory functions.Subsequently,the pathological changes of neurons in the hippocampus of rats were measured by HE staining,the protein expression of the GRP78,IRE1,XBP1,CHOP,caspase-3 and SIR in the hippocampus were measured by Western blot.Result s:Compared with the control group,the time of finishing the eight-arm radial maze prolonged and the number of total memory errors increased(P<0.05)in the RAM of the CUMS group rats.The morphology and structure of neurons in the hippocampus of CUMS group rats showed obvious pathological changes,the protein expression levels of GRP78,IRE1,XBP1,CHOP and caspase-3 significantly increased and SIR protein expression decreased(P<0.05)in hippocampus in the CUMS group compared with the control group rats.4-week treadmill exercise significantly improve the learning and memory ability and pathological changes of neurons in hippocampus of CUMS+TR group rats.Compared with CUMS group rats,the protein expression levels of GRP78,IRE1,XBP1,CHOP and caspase-3 significantly decreased,the SIR expression in hippocampus significantly increased in the CUMS+TR group rats(P<0.05).Conclusion:Treadmill exercise can improve cognitive impairment in CUMS rats,and its mechanism may be related to the up-regulation of SIR expression and the inhibition of ERS-mediated apoptosis pathway in hippo

关 键 词：跑台运动 慢性不可预知应激 认知障碍 sigma-1受体 内质网应激 凋亡 

分 类 号：R73[医药卫生—肿瘤]