启闭安神汤中活性成分对孤独症脂质代谢异常的作用  

The effects of active ingredients in Qi Bi Anshen decoction on abnormal lipid metabolism in autism

作　　者：范明磊 李佳敏 谢梦裴 杨奇 FAN Minglei;LI Jiamin;XIE Mengpei;YANG Qi(College of Life Science,Northwest University,Xi'an 710069;Department of Pharmacy,Tangdu Hospital,the Air Force Medical University,Xi'an 710038,China)

机构地区：[1]西北大学生命科学学院,西安710069 [2]空军军医大学唐都医院药剂科,西安710038

出　　处：《神经解剖学杂志》2025年第1期45-53,共9页Chinese Journal of Neuroanatomy

基　　金：陕西省重点研发计划(S2024-YF-YBSF-0941)。

摘　　要：目的:探究启闭安神汤(QAT)治疗孤独症(ASD)的有效活性成分,以及其对ASD行为和相关脂质代谢异常的影响。方法:24只成年雄性Sprague-Dawley大鼠随机分成3组:Control组、PPA组和PPA+QAT组,采用侧脑室注射丙酸法构建孤独症大鼠模型,QAT给药组连续灌胃7 d,行为学检测大鼠社交、重复刻板和焦虑样行为;超高液相色谱-串联质谱(UPLC-MS)分析大鼠差异代谢物及富集通路;网络药理学分析筛选QAT参与调节ASD的有效活性单体成分。10只性成熟C57BL/6J小鼠随机雌雄同笼配对,采用孕鼠单次腹腔注射丙戊酸钠法构建孤独症小鼠模型,孕鼠随机分为3组:Control组、VPA组和VPA+QUE组,给药组为孕鼠饮水中添加槲皮素(QUE),持续至围产期结束。行为学检测小鼠ASD样行为;试剂盒检测小鼠肝脏和血清中碱性磷酸酶(AKP)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)和总胆固醇(TC)含量,油红O染色观察肝脏细胞形态。结果:QAT给药后能够改善PPA诱导的ASD样行为(P<0.05);UPLC-MS分析各组大鼠差异代谢物主要富集在脂质代谢途径;网络药理学筛选出有效活性单体成分为QUE。QUE给药能够改善VPA诱导的ASD样行为(P<0.05);QUE给药能够逆转VPA诱导的肝脏中AKP、TC及TG的指标异常变化(P<0.05),减少肝脏中的脂滴沉积。结论:QAT中的活性单体成分QUE对ASD行为及相关肝脏脂质代谢异常具有治疗作用。Objective:To explore the effective active components of Qi Bi Anshen decoction(QAT)in the treatment of autism spectrum disorder(ASD)and its effects on ASD behaviors and related lipid metabolism abnormalities.Methods:24 adult male Sprague-Dawley rats were randomly divided into 3 groups:Control group,PPA group and PPA+QAT group.The ASD rat model was established by intracerebroventricular injection of propionic acid,and the QAT administration group was given intragastric administration for 7 days.Behavioral tests were conducted to detect the social,repetitive stereotyped and anxiety-like behaviors of rats.UPLC-MS was used to analyze the differential metabolites and enriched pathways of rats.Network pharmacology was used to screen the effective active monomer components of QAT involved in regulating ASD.10 sexually mature C57BL/6J mice were randomly paired in male-female cages.The ASD mouse model was established by a single intraperitoneal injection of sodium valproate to pregnant mice.The pregnant mice were randomly divided into 3 groups:Control group,VPA group and VPA+QUE group.The administration group was given QUE in the drinking water of pregnant mice until the end of the perinatal period.Behavioral tests were conducted to detect ASD-like behaviors in mice.Reagent kits were used to detect the contents of alkaline phosphatase(AKP),alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG)and total cholesterol(TC)in the liver and serum of mice.Oil red O staining was used to observe the morphology of liver cells.Results:QAT administration could improve the ASD-like behaviors induced by PPA(P<0.05).UPLC-MS analysis showed that the differential metabolites of each group of rats were mainly enriched in lipid metabolism pathways.Network pharmacology screening identified QUE as the effective active monomer component.QUE administration could improve the ASDlike behaviors induced by VPA(P<0.05).QUE administration could reverse the abnormal changes in AKP,TC and TG in the liver induced by VPA(P<0.05)an

关 键 词：启闭安神汤 孤独症谱系障碍 槲皮素 脂质代谢 

分 类 号：R285.5[医药卫生—中药学]