无创性迷走神经刺激对外伤性脑损伤模型大鼠的神经保护作用  

Neuroprotective effect of non-invasive vagus nerve stimulation on rats with traumatic brain injury

作　　者：梁勇 石佐林 郇宇 金海 LIANG Yong;SHI Zuolin;HUAN Yu;JIN Hai(Department of Neurosurgery,General Hospital of Northern Theater Command,Shenyang 110016,China)

机构地区：[1]中国人民解放军北部战区总医院神经外科,沈阳110016

出　　处：《神经解剖学杂志》2025年第1期54-58,共5页Chinese Journal of Neuroanatomy

基　　金：辽宁省应用基础研究计划(2022JH2/101500037)。

摘　　要：目的:探讨无创性迷走神经刺激(nVNS)对创伤性脑损伤(TBI)的神经保护作用。方法:利用侧位液压冲击法制备可控性皮质损伤(CCI)大鼠模型,进行nVNS治疗,利用平衡木实验检测大鼠的运动功能,通过大鼠脑组织的干湿比评估大鼠含水量,利用商品化试剂盒检测脑脊液中乳酸脱氢酶(LDH)的含量,利用RT-qPCR检测大鼠脑皮质中脑源性神经营养因子(BDNF)和神经生长因子(NGF)mRNA的表达。结果:经过nVNS刺激,CCI大鼠运动功能得到改善,脑含水量减少,脑脊液中LDH水平降低,同时脑皮质中BDNF和NGF mRNA表达上调。结论:nVNS通过对TBI大鼠具有神经保护作用,其机制可能与增加BDNF和NGF表达有关。Objective:To investigate the neuroprotective effect of non-invasive vagus nerve stimulation(nVNS)on traumatic brain injury(TBI).Methods:Male SD rats were used to prepare controlled cortical impact(CCI)rat model.nVNS treatment was performed.The motor function of rats was detected by beam walking test.The water content of rats was evaluated by the dry-wet ratio of rat brain tissue.The content of lactate dehydrogenase(LDH)in cerebrospinal fluid was detected by commercial kit.The expression of bain-derived neurotrophic factor(BDNF)and nerve growth factor(NGF)mRNA in rat cortex was detected by RT-qPCR.Results:After nVNS stimulation,CCI rats significantly improved the neurological function deficit,improved the motor ability,decreased the cerebral water content,decreased the level of LDH in cerebrospinal fluid,and upregulated the expression of BDNF and NGF mRNA in cortex.Conclusion:nVNS has a neuroprotective effect on TBI rats,and its mechanism may be related to the increased expression of BDNF and NGF.

关 键 词：无创性迷走神经刺激(nVNS) 创伤性脑损伤(TBI) 可控性皮质损伤(CCI) 脑水肿 神经营养因子 大鼠 

分 类 号：R74[医药卫生—神经病学与精神病学]