黄芩素通过AKT/GSK3β通路抑制脂多糖诱导的小胶质细胞活化  

Baicalein inhibits lipopolysaccharide-induced microglial activationvia the AKT/GSK3β pathway

作　　者：赵岩 徐辉[1] 付亚娜 岳圆圆 柴雪洁 李永征 ZHAO Yan;XU Hui;FU Yana;YUE Yuanyuan;CHAI Xuejie;LI Yongzheng(Qingdao Hiser Hospital Affiliated of Qingdao University,Qingdao Traditional Chinese Medicine Hospital,Qingdao 266000,China)

机构地区：[1]青岛大学附属青岛市海慈医院,青岛市中医医院,青岛266000

出　　处：《神经解剖学杂志》2025年第1期73-79,共7页Chinese Journal of Neuroanatomy

基　　金：山东省中医药科技项目(2021Z028)。

摘　　要：目的:探讨黄芩素对脂多糖(LPS)诱导活化的BV2小胶质细胞中蛋白激酶B(AKT)/糖原合成酶激酶3β(GSK3β)通路及肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)表达的影响。方法:培养BV2细胞株,分对照组(control)、LPS诱导组和LPS+黄芩素组(LPS+Baicalein)。分子对接(molecular docking)验证黄芩素与AKT的结合能力。免疫印迹(Western blot)及免疫荧光染色检测活化的BV2细胞中AKT、GSK3β、TNF-α、IL-1β的表达及AKT和GSK3β磷酸化水平(p-AKT和p-GSK3β)变化。结果:黄芩素与AKT具有较好的结合能力。Western blot结果显示,LPS诱导后,BV2细胞中TNF-α和IL-1β的表达升高,AKT和GSK3β磷酸化水平下降(P<0.05);黄芩素治疗后,与LPS组相比,TNF-α和IL-1β表达显著下降,AKT和GSK3β磷酸化水平升高(P<0.05)。免疫荧光染色结果与Western blot结果一致。结论:黄芩素抑制活化的小胶质细胞中TNF-α和IL-1β的表达,其作用可能与AKT/GSK3β通路的激活有关。Objective:To investigate the effects of baicalein on the protein kinase B(AKT)/glycogen synthase kinase 3β(GSK3β)pathway and the expression of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in lipopolysaccharide(LPS)-activated BV2 microglial cells.Methods:BV2 microglial cells were cultured and divided into control group,LPS-induced group,and LPS+Baicalein group.Molecular docking was conducted to verify the binding affinity of baicalein to AKT.Western blot and immunofluorescence staining were used to assess the expression and phosphorylation levels of AKT,GSK3β,TNF-α,and IL-1βin activated BV2 cells.Results:Baicalein exhibited a strong binding affinity for AKT.Western blot results showed that LPS stimulation led to increased TNF-αand IL-1βexpression and decreased phosphorylation of AKT and GSK3βin BV2 cells(P<0.05).After Baicalein treatment,TNF-αand IL-1βexpression significantly decreased,while AKT and GSK3βphosphorylation levels increased compared to the LPS group(P<0.05).Immunofluorescence staining results were consistent with those of Western blot.Conclusion:Baicalein inhibits the expression of TNF-αand IL-1βin activated microglia,potentially through activation of the AKT/GSK3βpathway.

关 键 词：黄芩素 脂多糖 AKT/GSK3β信号通路 BV2细胞 

分 类 号：R28[医药卫生—中药学]