基于LIF海水表层CDOM荧光特性仿真和实验研究  

Simulation and Experimental Study on the Fluorescence Characteristics of CDOM in Seawater Surface Based on LIF

作　　者：谢贝贝 王英杰 高旺 马凯杰 孔德明[3] XIE Bei-bei;WANG Ying-jie;GAO Wang;MA Kai-jie;KONG De-ming(School of Information Science and Engineering,Yanshan University,Qinhuangdao 066004,China;Key Laboratory for Computer Virtual Technology and System Integration of Hebei Province,Qinhuangdao 066004,China;School of Electrical Engineering,Yanshan University,Qinhuangdao 066004,China)

机构地区：[1]燕山大学信息科学与工程学院,河北秦皇岛066004 [2]河北省计算机虚拟技术与系统集成重点实验室,河北秦皇岛066004 [3]燕山大学电气工程学院,河北秦皇岛066004

出　　处：《光谱学与光谱分析》2025年第4期1116-1122,共7页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(62173289);河北省中央引导地方科技发展资金项目(236Z1704G);秦皇岛市科学技术研究与发展计划项目(202302B023)资助。

摘　　要：有色可溶性有机物(CDOM)作为全球碳循环和气候变化的关键物质,其荧光特性的研究对于海洋环境监测具有重要意义。激光诱导荧光(LIF)技术作为一种主动式的遥感光学探测手段,为CDOM的研究提供了有效工具。旨在通过仿真与实验相结合的方式,研究CDOM的荧光特性,为LIF探测海水表层CDOM提供理论基础。首先使用蒙特卡罗方法模拟海水表层CDOM的荧光辐射过程,对不同浓度CDOM、不同入射光天顶角、不同荧光接收角条件下CDOM的荧光仿真结果进行分析;然后通过实验搭建的LIF系统,探测以富里酸和腐殖酸为特征提取物配置的CDOM溶液,获得不同CDOM浓度、不同系统探测参量以及不同环境因素包括温度、 pH值和盐度的荧光光谱,分析荧光光谱特征,并使用单因素变化分析与多因素响应曲面分析结合的方式研究环境因素对CDOM荧光光谱的影响。仿真和实验结果表明CDOM荧光与激发光的入射天顶角成负相关,出射角度在0°~60°范围内的CDOM荧光强度较大,可为LIF实际探测CDOM时激发光的入射角度和接收角度的选择提供参考;随着CDOM浓度升高,荧光强度不断增加,到达一定浓度后,荧光强度随着浓度增加而减少;通过单因素变化分析研究了温度、 pH和盐度等环境因素对于CDOM的影响,结果表明随着温度上升,CDOM的荧光强度逐渐降低,当海水环境处于强碱状态下,CDOM的荧光光谱发生较大变化,在盐度对于CDOM的影响中,盐度对于富里酸的影响比对腐殖酸的影响大;然后使用响应曲面法分析多种因素共同作用下对于CDOM荧光特性的影响,根据观察CDOM荧光峰响应曲面的陡峭程度可以发现:各影响因素对于CDOM荧光强度的影响程度大小依次为pH、温度、盐度,这些结果说明利用LIF探测CDOM时需要考虑环境因素带来的影响。Colored dissolved organic matter(CDOM),a key substance in the global carbon cycle and climate change,is important in studying marine environmental monitoring for its fluorescence characteristics.Laser-induced fluorescence(LIF)technology,as an active remote sensing optical detection method,provides an effective tool for studying CDOM.This article aims to investigate the fluorescence characteristics of CDOM through a combination of simulation and experimentation,providing a theoretical basis for detecting CDOM in the surface layer of seawater using LIF.Firstly,the Monte Carlo method is utilized to simulate the fluorescence radiation process of CDOM in the surface layer of seawater.This approach allows for the analysis of fluorescence simulation results of CDOM under various conditions,including different concentrations of CDOM,incident light zenith angles,and fluorescence receiving angles.Subsequently,an LIF system is established through an experimental setup to detect CDOM solutions configured with fulvic acid and humic acid as characteristic extracts.This system s fluorescence spectra are obtained for different CDOM concentrations,system detection parameters,and environmental factors such as temperature,pH value,and salinity.The characteristics of these fluorescence spectra are then analyzed.Finally,the impact of environmental factors on the fluorescence spectra of CDOM is studied by combining single-factor variation analysis with multi-factor response surface analysis.Simulation and experimental results indicate a negative correlation between CDOM fluorescence and the incident zenith angle of excitation light.CDOM fluorescence intensity is relatively high when the emission angle is within the range of 0°~60°,which can provide a reference for the selection of incident and receiving angles of excitation light during the actual detection of CDOM using LIF;As the concentration of CDOM increases,the fluorescence intensity continues to rise.However,after reaching a certain concentration,the fluorescence intensity de

关 键 词：激光诱导荧光 有色可溶性有机物 荧光强度 环境因素 

分 类 号：P733.3[天文地球—物理海洋学]