山柰酚通过TGFBR1通路对Tca-83细胞恶性生物学行为的影响  

Effects of kaempferol on biological behaviors of Tca-83 cells via TGFBR1 signaling pathway

作　　者：周婷婷 李冬雅 陈卫芳[1] 汪声明 葛殿奎 ZHOU Ting-ting;LI Dong-ya;CHEN Wei-fang;WANG Sheng-ming;GE Dian-kui(Department of Stomatology,Huai'an Second People's Hospital.Huai'an 223000,Jiangsu Province,China)

机构地区：[1]淮安市第二人民医院口腔科,江苏淮安223000

出　　处：《中国口腔颌面外科杂志》2025年第2期105-109,共5页China Journal of Oral and Maxillofacial Surgery

摘　　要：目的:探讨山柰酚通过TGFBR1通路对Tca-83细胞恶性生物学行为的影响。方法:选择人舌鳞癌细胞系Tca-83细胞,用不同浓度山柰酚药液(25、50、100 mmol/L)处理,空白对照组不做药物处理。采用CCK-8实验评估Tca-83细胞的生长状况,通过流式细胞术分析细胞的凋亡状态和周期分布,使用Transwell小室技术评价山柰酚对Tca-83细胞侵袭能力和迁移能力的影响,采用实时荧光定量PCR检测凋亡相关因子Bcl-2、Bax、Caspase-3以及TGFBR1信号通路中TGFBR1、Smad 2/3 mRNA的表达变化。采用SPSS 22.0软件包对数据进行统计学分析。结果:随着山柰酚浓度的增加以及处理时间的延长,实验组细胞的增殖抑制效果逐渐增强,表现为浓度及时间依赖性。实验组G0/G1期、S期细胞比例与空白对照组相比有显著差异(P<0.05),细胞凋亡率较空白对照组显著增加(P<0.05)。与空白对照组相比,实验组的侵袭性和迁移性细胞数量显著减少(P<005),且减少的趋势随着山柰酚浓度的增加而更明显;实验组Bax、Caspase-3 mRNA表达量较空白对照组显著增高,Bcl-2 mRNA表达量显著下降(P<0.05)。各实验组TGFBR1及Smad 2/3 mRNA表达量均显著低于空白对照组(P<0.05),且随着山柰酚浓度的增加而降低(P<0.05)。结论:山柰酚对舌鳞癌Tca-83细胞的生物学效应包括抑制其增殖和迁移,促进细胞凋亡,可能与其抑制TGFBR1信号通路的激活有关。PURPOSE:To explore the effect of kaempferol on the malignant biological behavior of Tca-83 cells through TGFBR1 pathway.METHODS:Human tongue squamous cell line Tca-83 cells were treated with different concentrations of kaempferol solution(25,50,100 mmol/L)and normal saline,which were set as experimental group and blank control group,respectively.CCK-8 assay was used to assess the growth of Tca-83 cells.The apoptotic status and cycle distribution of these cells were analyzed by flow cytometry,the effect of kaempferol on the invasive and migration ability of Tca-83 cells was detected by Transwell assay.Real-time quantitative PCR was used to detect the changes of mRNA expression of Bcl-2,Bax,Caspase-3 and TGFBR1 signaling pathways associated with apoptosis.SPSS 22.0 software package was used for data analysis.RESULTS:With the increase of kaempferol concentration and the extension of treatment time,the proliferation inhibition effect of cells in the experimental group was gradually enhanced in concentration and time-dependent way.The proportion of cells arrested in G0/G1 and S phase showed significant difference between groups(P<0.05),and the experimental group had higher apoptosis rate than that of the blank control group(P<0.05).Compared with the blank control group,the experimental group had smaller number of invaded and migrated cells(P<0.05),and the number was gradually decreased with the increase of kaempferol concentrations.The mRNA expression of Bax and Caspase-3 in the experimental group was significantly higher than that in the blank control group,and the Bcl-2 mRNA expression was significantly lower than that in the blank control group(P<0.05).The mRNA expressions of TGFBR1 and Smad 2/3 in the experimental groups were significantly lower than those in the blank control group(P<0.05),and were decreased with the increase of kaempferol concentration(P<0.05).CONCLUSIONS:The biological effects of kaempferol on Tca-83 cells include inhibition of their proliferation and migration capacity and promoting apoptosis,

关 键 词：山柰酚 舌鳞癌 Tca-83细胞 TGFBR1通路 增殖 凋亡 迁移 

分 类 号：R739.8[医药卫生—肿瘤]