黄精多糖的树脂法脱色  

Resin Method for Decolorization of Polysaccharides of Polygonati Rhizoma

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作  者:郑艳宇 王平 刘思冶 郝明洋 赵思远 薛晓丽[3] ZHENG Yanyu;WANG Ping;LIU Siye;HAO Mingyang;ZHAO Siyuan;XUE Xiaoli(School of Biological and Pharmaceutical Engineering,Jilin Agricultural Science and Technology University,Jilin City 132100,China;Suzhou Eye Mist Medical Technology Co.,Ltd.,Suzhou 215000,China;Experimental Testing Center,Jilin Agricultural Science and Technology University,Jilin City 132100,China)

机构地区:[1]吉林农业科技学院生物与制药工程学院,吉林吉林132100 [2]苏州眼雾医疗科技有限公司,江苏苏州215000 [3]吉林农业科技学院实验检测中心,吉林吉林132100

出  处:《吉林化工学院学报》2024年第9期41-46,共6页Journal of Jilin Institute of Chemical Technology

摘  要:采用水提醇沉法制备黄精粗多糖,考察7种不同类型大孔吸附树脂对多糖的脱色效果。利用静态吸附确定最佳树脂,利用静态和动态单因素吸附试验优化树脂的脱色工艺。结果表明,D301G树脂对黄精多糖具有较好的综合脱色效率;静态吸附的最佳工艺参数:树脂用量120(g/mL),脱色时间6 h,初始pH=4,吸附温度40℃,该条件下多糖保留率为93.79%,多糖脱色率为68.54%;动态吸附的最佳工艺参数:上样多糖溶液浓度8 mg/mL,洗脱流速3.0 BV/h,径高比为18,多糖保留率为94.40%,多糖脱色率为78.73%。综上所述,D301G大孔吸附树脂可用于黄精多糖的脱色纯化,条件温和环保,有助于黄精多糖进一步的工业化产品开发。Polysaccharides of Polygonati Rhizoma was prepared by aqueous extraction and alcohol precipitation method,and the effect of seven macroporous adsorption resins on the decolorization of polysaccharides was investigated.The static adsorption was used to determine the optimal resin,and the static and dynamic one-factor adsorption tests were used to optimize the decolorization process of the resin.The results shown that the D301G resin had good decolorization efficiency for polysaccharides of Polygonati Rhizoma;the optimal process parameters for static adsorption were:resin dosage of 120(g/mL),decolorization time of 6 h,initial pH=4,and adsorption temperature of 40℃,and the retention rate of polysaccharides under these conditions was 93.79%,and the decolorization rate of polysaccharides was 68.54%.The optimal process parameters for dynamic adsorption were:polysaccharide solution concentration of 8,elution flow rate of 3.0 BV/h,diameter to height ratio of 18,polysaccharide retention of 94.40%and polysaccharide decolorization of 78.73%.In conclusion,D301G macroporous adsorbent resin can be used for the decolorization and purification of polysaccharides from Polygonati Rhizoma under mild and environmentally friendly conditions,which is helpful for the further development of Polygonati Rhizoma as an industrialized product.

关 键 词:黄精多糖 大孔吸附树脂 脱色评价 综合隶属度 

分 类 号:R284.2[医药卫生—中药学]

 

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