阿胶粉缓解体力疲劳的功能评价与机制探讨  

Function evaluation and mechanism of Ejiao powder in relieving physical fatigue

作　　者：韩香 郭晓飞 陆晓东 陈雪梅 胡博 王周平[1,2] HAN Xiang;GUO Xiaofei;LU Xiaodong;CHEN Xuemei;HU Bo;WANG Zhouping(School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China;Engineering Research Center for Functional Food,Jiangnan University,Wuxi,Jiangsu 214122,China;National Engineering Technology Research Center for Gelatin-based Traditional Chinese Medicine,Dong'e Ejiao Co.,Ltd.,Liaocheng,Shandong 252200,China;Liaocheng Key Laboratory of Functional Food Research and Development,Liaocheng,Shandong 252200,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学国家功能食品工程技术研究中心,江苏无锡214122 [3]国家胶类中药工程技术研究中心,山东聊城252200 [4]聊城市功能性食品研究开发重点实验室,山东聊城252200

出　　处：《食品与机械》2025年第2期121-127,共7页Food and Machinery

摘　　要：[目的]研究以驴皮、冰糖、黄酒为原料制备而成的阿胶粉缓解小鼠体力疲劳的活性机制。[方法]将50只SPF(specific pathogen free,SPF)级雄性BALB/c小鼠随机分为5组(每组10只),分别为空白阴性对照组、模型阳性对照组与低[0.67 g/kg]、中[1.33 g/kg]、高[2.67 g/kg]剂量的试验组。除对照组外,对其余组采用不同剂量的阿胶粉灌胃,连续灌胃30 d后,分别测定各组试验动物的负重游泳时间及运动后血清尿素、肌糖原、血乳酸、ATP的含量,同时对粪便样本中微生物菌群进行基因组测序。[结果]相对于模型对照组,低剂量阿胶粉组延长小鼠负重游泳时间显著(P<0.05),各剂量组小鼠运动后血清尿素存留量显著下降(P<0.01),中、高剂量组小鼠肌糖原的储备量有显著差异(P<0.01),小鼠血乳酸水平显著降低(P<0.01),中、高剂量组小鼠体内ATP的代谢显著提升(P<0.01);粪便样本中微生物菌群基因组测序的结果呈现出中剂量组>低剂量组>高剂量组>空白组;摄入低剂量和高剂量阿胶粉的小鼠肠道微生物菌群呈现出拟杆菌门含量的减少,而中剂量阿胶粉组的小鼠显著地呈现出厚壁菌属的增加,对应更好的碳水化合物和脂质代谢能力,能够为机体提供更多能量从而缓解体力疲劳。[结论]阿胶粉具有缓解小鼠体力疲劳的活性作用,并可以改善微生态失调和失衡。[Objective]To investigate the active mechanism of Ejiao powder,prepared from donkey-hide gelatin,rock sugar,and rice wine,in alleviating physical fatigue in mice.[Methods]Fifty specific pathogen-free(SPF)male BALB/c mice were randomly divided into five groups(10 in each group):a blank negative control group,a model positive control group,and three experimental groups receiving low(0.67 g/kg),medium(1.33 g/kg),and high(2.67 g/kg)doses of Ejiao powder.Except for the control group,all other groups were administered different doses of Ejiao powder via gavage for 30 consecutive days.Following administration,weight-bearing swimming time was recorded,and post-exercise serum urea,muscle glycogen,blood lactic acid,and ATP levels were measured.Additionally,the microbial community in fecal samples was analyzed through genome sequencing.[Results]Compared with the model control group,the low-dose Ejiao powder group exhibited a significant increase in weight-bearing swimming time(P<0.05).Serum urea levels after exercise were significantly reduced in all Ejiao-treated groups(P<0.01),while muscle glycogen reserves in the medium-and high-dose groups showed significant differences(P<0.01).Blood lactic acid levels were significantly decreased(P<0.01),and ATP metabolism was significantly enhanced in the medium-and high-dose groups(P<0.01).Genome sequencing of fecal microbiota revealed a ranking of microbial diversity as follows:medium-dose group>low-dose group>high-dose group>blank control group.Mice in the low-and high-dose Ejiao powder groups exhibited a reduction in Bacteroidetes,while those in the medium-dose group showed a significant increase in Firmicutes,which corresponded to improved carbohydrate and lipid metabolism,providing more energy to alleviate physical fatigue.[Conclusion]Ejiao powder demonstrated an active effect in alleviating physical fatigue in mice and improved microecological imbalance.

关 键 词：阿胶粉 抗疲劳 功效 评价 肠道菌群 基因组 

分 类 号：R28[医药卫生—中药学]