丝穗金粟兰根中1个新的乌药烷型倍半萜二聚体  

A new lindenane-type sesquiterpene dimer from the roots of Chloranthus fortunei

作　　者：边樱 谢小萍 张志君 BIAN Ying;XIE Xiao-ping;ZHANG Zhi-jun(School of Pharmacy,Hubei University of Science and Technology,Xianning 437100,China)

机构地区：[1]湖北科技学院药学院,咸宁437100

出　　处：《天然产物研究与开发》2025年第3期450-456,共7页Natural Product Research and Development

基　　金：咸宁市科技计划项目重点项目(2023SFYF069);湖北科技学院校内培育项目(2023-24X08)。

摘　　要：本研究旨在探究丝穗金粟兰根的倍半萜类成分及其抗炎活性。采用正相硅胶柱色谱、ODS柱色谱、SephadexLH-20柱色谱和半制备高效液相色谱等方法对丝穗金粟兰根的95%乙醇提取物进行分离纯化,并通过NMR、HR-ESI-MS、ECD等方法鉴定化合物结构。从丝穗金粟兰根中分离得到7个倍半萜二聚体,包括chlorantuforlide A(1)、chloramultilide C(2)、chlorahololide D(3)、chlorahololide B(4)、fortulactone A(5)、shizukaol C(6)、fortunilide C(7)。其中,化合物1是新的乌药烷型倍半萜二聚体,化合物2~4、6为首次从丝穗金粟兰中分离得到。活性实验表明化合物1能抑制脂多糖诱导的RAW 264.7细胞NO生成,表现出潜在的抗炎活性。This study aims to investigate the sesquiterpenoids from the root of Chloranthus fortunei and their anti-inflammatory activity.By using silica gel column,ODS column,Sephadex LH-20 gel column and semi-preparative HPLC,the 95%ethanol extract of the root of C.fortunei was isolated and purified.The structures of isolated compounds were elucidated by NMR,HR-ESI-MS,and ECD methods.Seven sesquiterpene dimer compounds were obtained from the root of C.fortunei,including chlorantuforlide A(1),chloramultilide C(2),chlorahololide D(3),chlorahololide B(4),fortulactone A(5),shizukaol C(6),and fortunilide C(7).Compound 1 was a new lindenane-type sesquiterpene dimer,and compounds 2-4 and 6 were isolated from C.fortunei for the first time.Activity experiment showed that compound 1 could significantly inhibit the production of NO in RAW 264.7 cells induced by lipopolysaccharide,showing potent anti-inflammatory activity.

关 键 词：丝穗金粟兰 倍半萜二聚体 抗炎活性 

分 类 号：R284.2[医药卫生—中药学]