大球盖菇—大豆—豆丹种养循环模式对土壤细菌群落结构和多样性的影响  

Effects of“Stropharia rugosannulata-Soybean-Clanis bilineata”Rotation on Bacterial Community Structure and Diversity in Soil

作　　者：苏文英 王一璞 刘晓梅 纪伟 孙潇潇 胡曙鋆 任立凯 SU Wen-ying;WANG Yi-pu;LIU Xiao-mei;JI Wei;SUN Xiao-xiao;HU Shu-yun;REN Li-kai(Lianyungang Academy of Agricultural Sciences,Lianyungang 222000,China;Lianyungang Crop Technical Guidance Station,Lianyungang 222000,China)

机构地区：[1]连云港市农业科学院,江苏连云港222000 [2]连云港市农作物技术指导站,江苏连云港222000

出　　处：《江西农业学报》2025年第3期47-53,62,共8页Acta Agriculturae Jiangxi

基　　金：江苏省现代农业(蔬菜)产业技术体系(JATS[2022]177);连云港市重点研发计划(SF2241)。

摘　　要：以普通种植模式(CK)及大球盖菇—大豆—豆丹种养循环模式(JZ)为研究对象,采用高通量测序技术对不同种植模式下的土壤理化性质、细菌群落的多样性和结构进行了测定分析,探讨了大球盖菇—大豆—豆丹种养循环模式对土壤细菌群落多样性及结构的影响。结果表明:JZ组的有机质、硝态氮、有效磷含量较CK明显增加,但EC值、速效钾、盐含量较CK明显降低。在2种种植模式下,土壤细菌的优势菌群为放线菌门、变形菌门、芽单胞菌门、酸杆菌门及绿弯菌门,种养循环模式后,土壤中放线菌门、绿弯菌门、奇古菌门的占比降低;变形菌门、芽单胞菌门、酸杆菌门的占比上升。Alpha多样性结果表明,CK的Chao1指数、ACE数、Shannon指数、Simpson指数均高于JZ组,说明在种养循环模式后,土壤中的细菌多样性降低。应用大球盖菇—大豆种植模式可提高土壤中的有机质含量和氮、磷含量,土壤细菌群落的丰度和多样性降低,但拟杆菌门、变形菌门、放线菌门等有益菌相对丰度的提高可以减少病害的发生。该研究结果可为优化土壤微生态调控机制及推进区域农业结构转型提供理论依据和数据支撑。Two planting modes,common planting mode(CK)and“Stropharia rugosannulata-Soybean-Clanis bilineata”breeding cycle mode(JZ),were employed as research subjects.High-throughput sequencing technology was used to determine and analyze the soil physical and chemical properties,bacterial community structure,and diversity under different planting methods.The impact of the cultivation cycle model of“Stropharia rugosannulata-Soybean-Clanis bilineata”on the diversity and structure of soil bacterial communities was explored.The results showed that the cultivation cycle of“Stropharia rugosannulata-Soybean-Clanis bilineata”significantly increased organic matter content,nitrate nitrogen levels,and available phosphorus,while significantly decreased electrical conductivity(EC),available potassium,and salinity.The predominant bacterial groups identified in the soil included Actinomycetes,Proteobacteria,Blastomonas,Acidobacteria and Chloromycetes.Under the cultivation cycle,there was a decrease in the proportion of Actinomycetes,Chloromycetes,and Archaea in the soil,while proportions of Proteobacteria,Blastomonas,and Acidobacteria increased.Alpha diversity analysis showed that Chao1 index values ACE number,shannon index and Simpson index were significantly higher in CK than those of JZ group samples,indicating a reduction in overall soil bacterial diversity post-breeding cycle mode.The rotation pattern of“Stropharia rugosannulata-Soybean-Clanis bilineata”increased organic matter,nitrogen and phosphorus contents in the soil but decreased both abundance and diversity in the overall soil bacterial community.Nevertheless,the relative abundance of beneficial bacteria such as Bacteroidetes,Proteobacteria and Actinomycetes may mitigate disease occurrence.The study results can provide foundational data for maintaining a healthy soil micro-ecological environment and facilitating regional agricultural structural adjustments.

关 键 词：种养循环 高通量测序 土壤细菌群落 农业结构调整 

分 类 号：S154.3[农业科学—土壤学]