高效液相色谱法测定膨化宠物食品中姜黄素类化合物的含量  

Determination of curcumin compounds in puffed pet food by high-performance liquid chromatography

作　　者：尤蕾 方绍明 黄福青 邱小风 钟顺 胡曼丽 张鑫 You Lei;Fang Shaoming;Huang Fuqing;Qiu Xiaofeng;Zhong Shun;Hu Manli;Zhang Xin(Gannan Medical University,Jiangxi Ganzhou 341000;Gannan Innovation and Transformation Medical Research Insitute,Jiangxi Ganzhou 341000;State Key Laboratory of New Targets Discovery and Drug Development for Major Disease,Jiangxi Ganzhou 341000;Ganzhou Pet Health Science Research Institute,Jiangxi Ganzhou 341000)

机构地区：[1]赣南医科大学,江西赣州341000 [2]赣南创新与转化医学研究院,江西赣州341000 [3]重大疾病新药靶发现及新药创制全国重点实验室,江西赣州341000 [4]赣州宠物大健康科学研究院,江西赣州341000

出　　处：《福建畜牧兽医》2025年第2期18-23,26,共7页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：国家自然科学基金项目(82260121);赣鄱俊才支持计划-主要学科学术和技术带头人培养项目-青年人才(20232BCJ23087)资助。

摘　　要：本研究旨在建立膨化宠物食品中姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素含量的高效液相色谱检测方法。使用C18色谱柱(250 mm×4.6 mm×5μm),流动相为乙腈:甲酸(0.1%)=65:35,提取剂为甲醇,流速为1.0 mL/min,柱温为30℃,检测波长为425 nm,进样量为10μL。检测姜黄素类化合物使用甲醇超声溶解30 min,过膜后上机检测。结果显示,该方法检测姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素分别在4.24~84.83μg/mL、1.70~68.00μg/mL、5.64~84.56μg/mL范围内线性良好,相关系数分别为0.9999、0.9991、0.9997。检出限分别为0.020μg/mL、0.013μg/mL、0.017μg/mL,定量限分别为0.080μg/mL、0.0544μg/mL、0.068μg/mL。该方法操作简单、成本低、准确度高、精密度良好,适用于膨化宠物食品中姜黄素类化合物含量的测定。An efficient high-performance liquid chromatography(HPLC)method was established for the determination of curcumin,demethoxycurcumin,and bisdemethoxycurcumin in dog feed.The HPLC was performed using a C18 column(250 mm×4.6 mm×5μm)with a mobile phase consisting of acetonitrile and 0.1%formic acid in a ratio of 65:35.Methanol was used as the extraction solvent,with a flow rate of 1.0 mL/min,a column temperature of 30°C,and a detection wavelength of 425 nm.A sample volume of 10μL was injected.The curcumin compounds were dissolved in methanol using ultrasonic extraction for 30 minutes,followed by filtration before analysis.The results indicated that the method exhibited good linearity for curcumin,demethoxycurcumin,and bisdemethoxycurcumin in the concentration ranges of 4.24~84.83μg/mL,1.70~68.00μg/mL,and 5.64~84.56μg/mL,respectively.The limits of detection were 0.020μg/mL,0.013μg/mL,and 0.017μg/mL,while the quantification limits were 0.080μg/mL,0.0544μg/mL,and 0.068μg/mL,respectively.This method is easy-operation,cost-efficient,highly accurate,and with excellent precision,which is proper for the determination of curcumin compounds in dog feed.

关 键 词：膨化宠物食品 姜黄素 去甲氧基姜黄素 双去甲氧基姜黄素 高效液相色谱法 

分 类 号：R28[医药卫生—中药学]