一株致驴腹泻大肠杆菌的分离鉴定  

Isolation and identification of a strain of Escherichia coli causing diarrhea in donkeys

作　　者：韩华 吴涛 王宁宁 刘雪松 李莉 吴宪 陈亮 徐婷婷 兰世捷 张艳 江波涛 Han Hua;Wu Tao;Wang Ningning;Liu Xuesong;Li Li;Wu Xian;Chen Liang;Xu Tingting;Lan Shijie;Zhang Yan;Jiang Botao(Heilongjiang Province Animal Quarantine Center,Harbin 150000;Heilongjiang Province Animal Disease Prevention and Control Center,Harbin 150000;Branch of Animal Husbandry and Veterinary,Heilongjiang Academy of Agricultural Sciences,Heilongjiang Qiqihar 161005;Heilongjiang Key Laboratory of Veterinary Drugs,Heilongjiang Qiqihar 161005)

机构地区：[1]黑龙江省动物检疫中心,哈尔滨150000 [2]黑龙江省动物疫病预防与控制中心,哈尔滨150000 [3]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005 [4]黑龙江省兽用药物重点实验室,黑龙江齐齐哈尔161005

出　　处：《福建畜牧兽医》2025年第2期24-26,共3页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：黑龙江省农业科技创新跨越工程农业特色产业科技创新支撑项目(CX23TS21);黑龙江省省属科研院所科研业务费项目(CZKYF2025-1-B011)资助。

摘　　要：某地区规模化养驴场出现驴腹泻现象,对该场病死驴的肝脏及肠道内容物进行样本采集,并开展细菌分离培养。采用分子生物学技术对分离的菌株进行深入分析。研究结果表明,从病死驴的肝脏中分离到1株革兰氏阴性杆菌;通过16S rRNA测序确认该菌株为大肠杆菌。小鼠致病性试验显示该菌株具有致病性;毒力基因筛查结果表明,该株携带STa和K88毒力基因;药敏试验结果显示,该大肠杆菌对4种抗菌药物敏感,但对另外4种抗菌药物则表现出耐药性。本研究成功分离并鉴定出1株驴源致病性大肠杆菌,并对其毒力基因及耐药性进行分析,为防治由该菌株引起的驴腹泻提供了重要的科学依据。In a certain area,a large-scale donkey farm reported cases of diarrhea among its donkeys.Samples were collected from the livers and intestinal contents of deceased donkeys,and bacterial isolation and culture were performed.Subsequently,molecular biology techniques were used for in-depth analysis of the isolated strains.The results indicated the isolation of a Gram-negative bacillus from the liver of a deceased donkey,which was confirmed as Escherichia coli through 16S rRNA sequencing.Pathogenicity experiments in mice demonstrated that this strain was pathogenic.Virulence gene screening revealed that the strain carried the STa and K88 virulence genes.Antibiotic susceptibility testing showed that this strain of E.coli was sensitive to four antimicrobial agents but exhibited resistance to four others.This study successfully isolated and identified a pathogenic strain of E.coli,analyzing its virulence genes and antibiotic resistance,thus providing important scientific evidence for the prevention and control of diarrhea in donkeys caused by this strain.

关 键 词：驴 腹泻 大肠杆菌 毒力基因 

分 类 号：S85[农业科学—兽医学]