黄芪多糖联合维生素C对镉致雌性大鼠肾脏损伤的保护作用  

Protective effect of Astragalus polysaccharide combined with vitamin C on cadmium induced kidney injury in female rats

作　　者：王俊诸 郭威[1] 范曦 黄强 杨俊 陈蓉[1] WANG Junzhu;GUO Wei;FAN Xi;HUANG Qiang;YANG Jun;CHEN Rong(Changsha Medical University,Changsha 410219,China;The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China)

机构地区：[1]长沙医学院,湖南长沙410219 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《陕西医学杂志》2025年第4期458-464,共7页Shaanxi Medical Journal

基　　金：国家级大学生创新训练计划项目(教高司函[2023]8号-S202310823014);湖南省大学生创新创业训练计划项目(湘教通[2023]237号-4016);湖南省教育厅科学研究项目(20C0179)。

摘　　要：目的:探究黄芪多糖联合维生素C对镉致雌性大鼠肾脏损伤的保护作用。方法:将24只SD雌性大鼠随机分成四组每组6只,分别为正常对照组、镉染毒组、黄芪多糖联合维生素C低浓度组、黄芪多糖联合维生素C高浓度组。试验结束后处死大鼠采集新鲜血液,生物化学法检测大鼠肾功能生化指标肌酐(CRE)含量、尿素氮(BUN)含量,并采集其肾脏组织进行苏木精-伊红染色法(HE)观察肾组织病理变化情况;马松染色法(Masson)观察肾组织纤维化情况;免疫荧光法(IF)、免疫组化法(IHC)检测肾组织中半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白表达情况;原位末端标记法(TUNEL荧光染色)检测肾组织细胞凋亡情况。结果:经氯化镉长期腹腔注射后,镉染毒组雌性大鼠生化结果显示血清中CRE、BUN含量显著升高;HE结果显示可见少量肾小管上皮细胞水肿,细胞肿胀,胞质疏松淡染,间质明显增生,可见少量炎性细胞浸润,肾小体形态发生不规则改变,肾小囊与肾小球之间界限模糊,球囊粘连;Masson结果显示肾脏组织可见大量蓝色胶原纤维;IF、IHC结果均显示凋亡信号相关通路Caspase-3的表达显著升高;TUNEL荧光染色结果显示肾脏组织细胞TUENL阳性表达率显著升高,肾脏组织细胞出现大量凋亡。较镉染毒组比较黄芪多糖联合维生素C低、高浓度组血清中CRE、BUN含量、凋亡信号相关通路Caspase-3的表达显著降低;病理损伤、肾脏组织纤维化、肾脏组织细胞凋亡等方面均出现不同程度的改善。结论:黄芪多糖联合维生素C对镉致雌性大鼠肾脏组织的损伤具有保护作用,可有效拮抗镉对肾脏组织的损伤,减少肾脏组织细胞的凋亡并恢复肾脏功能。Objective:To investigate the protective effect of Astragalus polysaccharides combined with vitamin C on cadmium induced kidney injury in female rats.Method:24 female SD rats were randomly divided into 4 groups,with 6 rats in each group:normal control group,cadmium exposure group,low concentration group of Huangqi polysaccharide combined with vitamin C,and high concentration group of Huangqi polysaccharide combined with vitamin C.After the experiment,the rats were euthanized and fresh blood was collected.Biochemical methods were used to detect the biochemical indicators of creatinine(CRE)and urea nitrogen(BUN)in the kidney function of the rats,and their kidney tissue was collected for hematoxylin and eosin(HE)staining to observe the pathological changes in the kidney tissue;Observation of renal tissue fibrosis using Masson staining method(Masson);Immunofluorescence assay(IF),immunohistochemistry(IHC),and detection of Caspase-3 protein expression in renal tissue;Detection of renal tissue cell apoptosis using in situ end labeling(TUNEL fluorescence staining)method.Results:After long-term intraperitoneal injection of cadmium chloride,the biochemical results of female rats in the cadmium exposed group showed a significant increase in serum CRE and BUN levels;The H&E results showed that a small amount of renal tubular epithelial cells were edematous,swollen,with loose and lightly stained cytoplasm and significant interstitial proliferation.A small number of inflammatory cell infiltration was observed,and irregular changes in renal corpuscle morphology were observed.The boundary between the renal capsule and glomerulus was blurred,and balloon adhesions were observed;the Masson results showed a large number of blue collagen fibers in the kidney tissue;Both IF and IHC results showed a significant increase in the expression of the apoptotic signaling pathway Caspase-3;the TUNEL fluorescence staining results showed a significant increase in the positive expression rate of TUNEL in renal tissue cells,and a large amount of ap

关 键 词：镉 黄芪多糖 维生素C 肾脏 凋亡 半胱氨酸天冬氨酸蛋白酶-3 

分 类 号：R692[医药卫生—泌尿科学]