温通活血乳膏对2型糖尿病周围神经病变大鼠核因子-E2相关因子2的影响  

Wentong Huoxue cream for type 2 diabetic peripheral neuropathy effect of Nrf2 in rats

作　　者：李惠[1] 李寅辉[1] 徐利娟 马丽[1] LI Hui;LI Yinhui;XU Lijuan;MA Li(Department of Endocrine,Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属中医医院内分泌科,新疆乌鲁木齐830000

出　　处：《陕西中医》2025年第4期445-449,共5页Shaanxi Journal of Traditional Chinese Medicine

基　　金：新疆维吾尔自治区自然科学基金-青年基金资助项目(2021D01C239)。

摘　　要：目的:通过温通活血乳膏干预糖尿病周围神经病变(DPN)大鼠模型,观察其对核因子-E2相关因子2(Nrf2)氧化应激通路的影响。方法:成年Wistar雄性大鼠36只,取其中6只作为空白组,30只建立DPN模型。将造模成功的30只大鼠随机分模型组、基质组、二甲双胍组及温通活血乳膏中、高剂量组;空白组及模型组不进行药物干预,基质组予以温通活血乳膏的基质干预,二甲双胍组给予二甲双胍干预,温通活血乳膏中、高剂量组分别给予中、高剂量温通活血乳膏。末次给药后,采用酶联免疫吸附实验(ELISA)检测血清髓过氧化物酶(MPO)、肿瘤坏死因子-α(TNF-α)水平,Western blot检测坐骨神经节中Nrf2、NF-κB蛋白表达,RT-PCR法检测Nrf2、NF-κB mRNA的表达。结果:温通活血乳膏可以降低MPO、TNF-α、NF-κB蛋白及mRNA的表达,增加Nrf2蛋白及mRNA的表达(均P<0.05),且温通活血乳膏高剂量组改善效果更显著。结论:温通活血乳膏可以通过上调Nrf2表达,抑制NF-κB表达,改善氧化应激,发挥治疗DPN的作用。Objective:Wentong Huoxue cream was used to intervene diabetic peripheral neuropathy(DPN)rat model to observe its effect on nuclear factor E2-related factor 2(Nrf2)oxidative stress pathway.Methods:Among 36 adult Wistar male rats,6 rats were used as blank group and 30 rats were used as DPN model.The 30 rats were randomly divided into model group,matrix group,metformin group,medium and high dose groups of Wentong Huoxue cream.No drug intervention was given to blank group and model group,matrix group was given matrix intervention of Wentong Huoxue cream,metformin group was given metformin intervention,Wentong Huoxue cream medium and high dose groups were given medium and high dose Wentong Huoxue cream,respectively.After the last dose,serum levels of myeloperoxidase(MPO)and tumor necrosis faction-α(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA).Expressions of Nrf2 and NF-κB protein in sciatic ganglia were detected by Western blot.And expressions of Nrf2 and NF-κB mRNA were detected by RT-PCR.Results:Wentong Huoxue cream could decrease the expression of MPO,TNF-α,NF-κB protein and mRNA,and increase the expression of Nrf2 protein and mRNA(all P<0.05),and the improvement effect of high dose Wentong Huoxue cream was more significant.Conclusion:Wentong Huoxue cream can inhibit the expression of NF-κB,improve the oxidative stress and play a role in the treatment of DPN by up-regulating the expression of Nrf2.

关 键 词：糖尿病周围神经病变 温通活血乳膏 大鼠 核因子-E2相关因子2 氧化应激 髓过氧化物酶 

分 类 号：R587.2[医药卫生—内分泌]