高温胁迫对膨腹海马幼苗存活率及抗氧化酶活性的影响  

Effects of High Temperature Stress on Survival Rate and Antioxidant Enzyme Activity of Hippocampus abdominalis Seedlings

作　　者：肖文杰 郭宝英 赵超 薛月光 刘长琳[2] 姜达 司仲强 XIAO Wen-Jie;GUO Bao-Ying;ZHAO Chao;XUE Yue-Guang;LIU Chang-Lin;JIANG Da;SI Zhong-Qiang(Zhejiang Ocean University,Zhoushan 316022;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071;Qingdao Qingyuan Marine Biotechnology Co.,LTD,Qingdao 266000;Qingdao Huangdao District Xuejia Island sub-district Office of agriculture,Qingdao 266000,China)

机构地区：[1]浙江海洋大学,舟山316022 [2]中国水产科学研究院黄海水产研究所,青岛266071 [3]青岛清源海洋生物科技有限公司,青岛266000 [4]青岛市黄岛区薛家岛街道办事处农业办,青岛266000

出　　处：《动物学杂志》2025年第1期59-68,共10页Chinese Journal of Zoology

基　　金：中国水产科学研究院基本科研业务费(No.2023TD30)。

摘　　要：为探明高温胁迫对膨腹海马(Hippocampus abdominalis)幼苗的影响,对20日龄幼苗进行24 h急性高温胁迫,测定其在不同温度和时间下的存活率及抗氧化酶活性。将幼苗从18℃直接转移到22、24、25、26、27、28和30℃,每隔2 h观察死亡数和行为状态,构建时间-温度-存活率模型。通过Probit回归分析,得出高起始致死温度(24 h-UILT50)。根据高起始致死温度结果,将幼苗从18℃直接转移到21、24和27℃,持续胁迫24 h,分别在应激0、3、6、12和24 h取样,用生化试剂盒测定不同温度和不同时间点的抗氧化酶活性。结果显示,膨腹海马幼苗的存活率随水温的升高不断降低,其高起始致死温度为27.53℃。在24 h急性高温胁迫过程中,21℃和24℃下超氧化物歧化酶(SOD)及过氧化氢酶(CAT)活性随着时间呈现先降后升的趋势;27℃下,超氧化物歧化酶和过氧化氢酶活性均呈升高趋势,24 h时显著高于对照组(18℃组)(P<0.05)。各温度组总抗氧化能力(T-AOC)的活性随时间均表现出升高-降低-升高的变化,且活性在12h时均降至最低。所有处理组丙二醛(MDA)的含量随时间均呈现升高的趋势,但24℃处理组于胁迫6 h后开始降低。实验表明,急性高温胁迫使膨腹海马幼苗抗氧化酶活性产生了显著变化,其体内抗氧化酶活性被激活用于消除高温应激产生的活性氧自由基(ROS),幼苗体内丙二醛含量显著增加,表明膨腹海马幼苗仍处于氧化应激状态。27℃处理组抗氧化酶活性变化趋势与其余温度处理组间均存在明显差异,表明膨腹海马幼苗无法适应27℃的高温。[Objectives]To investigate the effects of high temperature stress on Hippocampus abdominalis seedlings,20-day-old seedlings were subjected to acute high temperature stress for 24 h,and the survival rate and antioxidant enzyme activity were measured at different temperatures and times.[Methods]The seedlings were transferred directly from 18℃to 7 temperature gradients of 22,24,25,26,27,28 and 30℃,and the number of deaths and behavioral status were recorded every 2 h,and a time-temperature-survival model was constructed.Probit regression analysis was used to determine the temperature at which the mortality rate reached 50%in 24 h,which was considered the high initial lethal temperature(24 h-UILT50).According to the results,the seedlings were directly transferred from 18℃to 21℃,24℃and 27℃for 24 h.Samples were collected at stress 0,3,6,12 and 24 h,respectively,and the antioxidant oxidase activities at different temperatures and different time points were measured using biochemical kits.SPSS 26 software was used for data processing,and one-way ANOVA and Duncan test were used for significance analysis.[Results]The results showed that in the high initial lethal temperature experiment,the H.abdominalis seedlings did not die between 18﹣25℃,they began to die slightly at 26℃,and died completely at 30℃,and the survival rate decreased with the increase of water temperature.Probit regression analysis showed that the high initial lethal temperature was 27.53℃(Fig.1,Table 1).The activities of superoxide dismutase(SOD)and catalase(CAT)showed the same trend in the 24 h high temperature stress experiment.At 21℃and 24℃,the activities of SOD and CAT first decreased and then increased.The activities of SOD and CAT increased at 27℃,and were significantly higher than those of control group at 24 h(P<0.05)(Figs.2,3).The content of malondialdehyde(MDA)increased at the initial stage of stress,and did not change significantly at 21℃.After 6 h of stress,the content of MDA began to decrease in the 24℃treatment g

关 键 词：膨腹海马 高温胁迫 存活率 抗氧化酶 丙二醛 

分 类 号：Q955[生物学—动物学]