湖南城步发现隐耳蟾蜍  

Bufo cryptotympanicusin Found in Chengbu,Hunan,China

作　　者：肖蓓 李辉[1] 朱乐强 唐旭芬 张荣华 兰晓明 莫小阳[1,2] XIAO Bei;LI Hui;ZHU Le-Qiang;TANG Xu-Fen;ZHANG Rong-Hua;LAN Xiao-Ming;MO Xiao-Yang(Vertebrate Zoology Laboratory,College of Life Science,Hunan Normal University,Changsha 410081;College of Biological and Food Engineering,Huaihua University,Huaihua 418000;Nanshan National Park Administration of Hunan Province,Shaoyang 422500,China)

机构地区：[1]湖南师范大学生命科学学院脊椎动物学实验室,长沙410081 [2]怀化学院生物与食品工程学院,怀化418000 [3]湖南省南山国家公园管理局,邵阳422500

出　　处：《动物学杂志》2025年第1期105-114,共10页Chinese Journal of Zoology

摘　　要：2024年3月24日,在湖南省城步县南山国家公园黑山岭近溪流区(26°09′12″N,110°09′17″E,海拔1760 m),新采集到蟾蜍样本4号(标本号:HNNU2024032401~HNNU2024032404)。其形态特征为:雄性,耳旁腺明显,无鼓膜、无耳柱骨,背面皮肤光滑,瘰粒小而稀少,后肢较短且左右跟部不相遇,趾蹼不发达,与隐耳蟾蜍(Bufo cryptotympanicus)形态特征基本相符。基于16SrRNA与COI基因联合序列(1140 bp)的分子系统发育分析显示,这4号标本与模式产地隐耳蟾蜍聚为一支,遗传距离为0.2%,小于蟾蜍属(Bufo)平均遗传距离4.35%。确定本次所采集标本为蟾蜍科蟾蜍属的隐耳蟾蜍,为湖南省两栖动物分布新记录种。In order to investigate the biodiversity of amphibian and reptile in the Nanshan National Park,Chengbu County,Hunan Province,four specimens of the Bufo genus were collected(specimen numbers:HNNU2024032401﹣HNNU2024032404)near a stream in the Jintongshan area(26°09′12″N,110°09′17″E,altitude 1760 m)in April 2024.The specimens were identified based on morphological characteristics described by Nihn et al.(2022)and Fei et al.(2009).Two mitochondrial genes,namely 16S ribosomal RNA gene(16S rRNA)and cytochrome C oxidase I gene(COI),were concatenated into a single sequence of 1140 bp for phylogenetic analysis.Other sequences were obtained from GenBank(Appendix 1).DNA sequences were aligned in MEGA 7 by the Clustal W algorithm with default parameters.The dataset was partitioned according to genes and codons for 16S rRNA and COI,then tested in PhyloSuite 1.2.2,resulting in the best-fitting nucleotide substitution models of TIM2+I+G.Sequenced data were analyzed using Bayesian inference(BI)and maximum likelihood(ML)approaches.Two independent runs were conducted in the BI analysis with 2000000 generations each,and sampled every 1000 generations with the first 25%of samples discarded as burn-in.In the ML analysis,a bootstrap consensus tree was generated from 1000 replicates.Both the ML and BI analyses yielded essentially identical topologies,pairwise distances were calculated in MEGA 7 using the uncorrected model based on 16S rRNA sequences among all species used in this study,see details in Appendix 2.The morphological measurements of these specimens were displayed in Table 1,morphological characteristics include tympanum absent,dorsal surface of head and body smooth,within small and sparse granules,heels not meeting when flexed hind-limbs to body axis,and undeveloped toe pads,which are largely consistent with B.cryptotympanicus(Fig.2).Further phylogenetic analysis revealed that the specimens clustered within a branch of B.cryptotympanicus.Based on the uncorrected distance model of the 16S rRNA gene,the average ge

关 键 词：蟾蜍属 湖南省 分子发育分析 分布新记录 南山国家公园 

分 类 号：Q959[生物学—动物学]