链霉菌ZH-356的植病生防作用  

Biocontrol effects of Streptomyces sp.ZH-356 on plant diseases

作　　者：刘安东 宁婉清 朱旭飞 张博琳 屈小娜 张晋龙 徐成楠 李广伟[1] 张向前[1] 王延峰[1] 成娟丽 林金水 LIU Andong;NING Wanqing;ZHU Xufei;ZHANG Bolin;QU Xiaona;ZHANG Jinlong;XU Chengnan;LI Guangwei;ZHANG Xiangqian;WANG Yanfeng;CHENG Juanli;LIN Jinshui(Shaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau,College of Life Sciences,Yan'an University,Yan'an,Shaanxi,China;Yan'an Colony Biological Technology Co.,Ltd.,Yan'an,Shaanxi,China;State Key Laboratory for Crop Stress Resistance and High-efficiency Production,Northwest A&F University,Yangling,Shaanxi,China)

机构地区：[1]延安大学生命科学学院,陕西省黄土高原资源植物研究与利用重点实验室,陕西延安 [2]延安柯龙尼生物科技有限公司,陕西延安 [3]西北农林科技大学,作物抗逆与高效生产全国重点实验室,陕西杨凌

出　　处：《微生物学报》2025年第3期1219-1240,共22页Acta Microbiologica Sinica

基　　金：国家自然科学基金(32070103);作物抗逆与高效生产全国重点实验室开放课题(SKLCSRHPKF12);陕西省“特支计划”区域发展人才项目(2020-44);陕西省普通高等学校青年杰出人才支持计划(2018-111);陕西高校青年创新团队(2022-943);陕西省“两链”融合重点专项(2023LLRH-01);延安市科技计划(2024-JBZ-001);延安大学科研计划(2023HBZ-001,2023CGZH-007);陕西省大学生创新创业训练项目(S202310719112)。

摘　　要：【目的】对具有拮抗植物病原真菌作用的链霉菌ZH-356进行定殖能力和生防作用评价,并结合组学分析初步揭示其植病生防机制。【方法】采用绿色荧光蛋白(green fluorescent protein,GFP)荧光标记法,检测链霉菌ZH-356在植物体内的定殖情况;同时,通过测定不同剂型菌剂(种子包衣剂、可湿性粉剂、胶水菌剂和骨胶菌剂)对植物真菌病害的生防效果来评价链霉菌ZH-356的植病生防作用及潜力;利用三代测序技术分析链霉菌ZH-356的全基因组信息,并对其基因功能进行注释;结合转录组学对比分析链霉菌ZH-356在拮抗植物病原真菌过程中表达发生显著改变的基因,预测其拮抗活性物质的合成基因。【结果】通过GFP荧光标记的植物定殖分析,发现链霉菌ZH-356能在番茄和小麦的根茎中稳定定殖;同时,基于ZH-356制备的不同剂型菌剂对番茄早疫病和苹果树腐烂病均具有良好防效作用。其中,链霉菌ZH-356制成的种子包衣剂处理后不影响番茄种子的发芽率,且能有效保护番茄苗免受番茄早疫病菌的侵染;基于ZH-356制备的可湿性粉剂对番茄早疫病具有良好的预防与治疗效果,且预防作用优于治疗作用;由链霉菌ZH-356制成的液体菌剂,无论是否刮除发病处的树皮,对苹果树腐烂病均具有一定的防治效果,刮除病斑上的树皮后防治效果更好,且这种防治效果优于甲基硫菌灵农药。通过对链霉菌ZH-356的全基因组测序分析发现,其基因组含有一条线状染色体,大小为9 435 898 bp,G+C含量平均为70.82%,共预测到8 432个编码基因、69个t RNA基因和18个rRNA基因。物种注释结果显示,链霉菌ZH-356不属于任何已完成基因组测序的链霉菌种类。经全基因组分析预测,链霉菌ZH-356中含有32个次级代谢产物合成基因簇。通过转录组学分析发现,在拮抗植物病原真菌过程中,NRPS/T1PKS基因簇ZH_356_GM000343-ZH_356_GM000422的表达显著上调,推�[Objective]To evaluate the colonization ability and biocontrol effects of Streptomyces sp.ZH-356 with antagonistic effects on plant pathogenic fungi and reveal the biocontrol mechanism of Streptomyces sp.ZH-356 by omics analysis.[Methods]The colonization of Streptomyces sp.ZH-356 in plants was detected by the GFP fluorescent labeling method.The biocontrol effects and potential of Streptomyces sp.ZH-356 on plant fungal diseases were evaluated based on the biocontrol effects of the inoculant in different dosage forms(seed coating agent,wettable powder,gum inoculant,and bone glue inoculant).The whole genome information of Streptomyces sp.ZH-356 was analyzed by third-generation sequencing,and its gene functions were annotated.The comparative transcriptome analysis was performed to screen the differentially expressed genes during the antagonizing process of Streptomyces sp.ZH-356 against plant pathogenic fungi,and thus the genes involved in the synthesis of antagonistic substances were predicted.[Results]Streptomyces sp.ZH-356 stably colonized the roots and stems of tomato and wheat plants.Different dosage forms of inoculants prepared based on Streptomyces sp.ZH-356 demonstrated strong control effects on tomato early blight and apple valsa canker.Among them,the seed coating agent prepared with Streptomyces sp.ZH-356 did not affect the germination rate of tomato seeds after treatment while protecting tomato seedlings from the infection of Alternaria solani.The wettable powder prepared with Streptomyces sp.ZH-356 showed both prevention and treatment effects on tomato early blight,with the prevention effect stronger than the treatment effect.The liquid inoculants prepared from Streptomyces sp.ZH-356 had control effects on apple Valsa canker,regardless of whether the diseased bark was scraped or not,while the control effect was better when the diseased bark was scraped and better than that of thiophanate-methyl.The whole genome sequencing results showed that Streptomyces sp.ZH-356 contained only one linear chromosome with

关 键 词：链霉菌 定殖 菌剂 基因组测序 转录组测序 次级代谢产物 

分 类 号：S476[农业科学—农业昆虫与害虫防治]