氨基化碳量子点荧光猝灭-恢复体系构建与优化  

Contruction and optimization of fluorescence quenching-recovery of amino-functional carbon quantum dots

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作  者:杨艳楠 肖新才[1] 赵丹[1] YANG Yannan;XIAO Xincai;ZHAO Dan(School of Pharmacy,South-Central Minzu University,Wuhan 430074,China)

机构地区:[1]中南民族大学药学院,武汉430074

出  处:《华中师范大学学报(自然科学版)》2025年第2期220-227,共8页Journal of Central China Normal University:Natural Sciences

基  金:国家自然科学基金项目(21978329);中央高校基本科研业务费专项资金项目(CZP20004).

摘  要:碳量子点(CQDs)是一种新型荧光碳纳米材料,因其出色的生物相容性和卓越的光学特性而备受关注,但在其使用过程中的猝灭影响了监控效果.该文通过水热法合成碳量子点并用氨水使其表面氨基化,制备得到氨基化碳量子点(NH_(2)-CQDs),组建了NH_(2)-CQDs与聚丙烯酸(PAA)的荧光猝灭体系,用氢氧化钠(NaOH)定量探索体系的荧光恢复情况.采用透射电镜(TEM)、傅里叶红外光谱(FTIR)、紫外-可见光谱(UV-Vis)和荧光发射光谱(FL)分别进行NH_(2)-CQDs尺寸、成分和荧光强度的表征.重点考察了NaOH与PAA物质的量的关系和孵育时间对反应体系的影响.结果表明:NH_(2)-CQDs的平均粒径为4.8nm,表面含有羟基和含氮官能团等亲水性基团,在水溶液中有很好的水溶性和稳定性.NH_(2)-CQDs在332nm处有一明显吸收峰;当激发波长为378nm,发射波长为451nm处的荧光强度最大且该碳量子点具有激发波长依赖性,荧光发射峰的位置随激发波长的变化而变化.在PAA存在下可以有效地猝灭NH_(2)-CQDs荧光;而当有NaOH存在时荧光恢复.在含4.17×10^(-5)molPAA的猝灭体系中加入5.00×10^(-5)molNaOH荧光恢复程度最好(接近mPAA∶mNaOH=1∶1),且该体系具有较强的稳定性和可逆性,在最佳用量下观察聚合物整体轮廓清晰.该研究为碳量子点在线标记聚合物提供了可能.Carbon quantum dots(CQDs)is a novel type of fluorescent carbon nanomaterials,highly regarded for their excellent biocompatibility and outstanding optical properties.However,the fluorescence quenching during their application have affected the monitoring effect.In this study CQDs were synthesized through hydrothermal method and the surface of CQDs was amino modificated using ammonia(NH_(3)),obtaining amino-functional carbon quantum dots(NH_(2)-CQDs).A fluorescence quenching system comprising NH_(2)-CQDs and polyacrylic acid(PAA)was constructed,and the fluorescence recovery was quantitatively investigated using sodium hydroxide(NaOH).The characterization of NH_(2)-CQDs size,composition,and fluorescence intensity was conducted using transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR),ultraviolet-visible spectroscopy(UV-Vis),and fluorescence spectroscopy(FL).The relationship between the molar ratios of NaOH and PAA and the effect of incubation time on the reaction system were examed primarily.The results indicated that NH_(2)-CQDs had an average particle size of 4.8 nm,and their surfaces were rich in hydrophilic groups such as hydroxyl and nitrogen(N)-containing functional groups,offering excellent water solubility and stability in aqueous solutions.NH_(2)-CQDs exhibited a distinct absorption peak at 332 nm,with the highest fluorescence intensity at an emission wavelength of 451 nm when excited at 378 nm,and their emission peak position varies with the excitation wavelength.Under the presence of PAA,the fluorescence of NH_(2)-CQDs was effectively quenched;whereas under the presence of NaOH,the fluorescence recovered.The best fluorescence recovery was observed when 5.00×10^(-5)mol NaOH was added to the fluorescence quenching system containing 4.17×10^(-5)mol PAA(approximately m PAA∶m NaOH=1∶1),with relatively stronger stability and reversibility and demonstrating a clear overall contour of the polymer under the optimal conditions.This research potentially enables the online labeli

关 键 词:荧光猝灭-恢复 氨基化碳量子点 聚丙烯酸 标记 

分 类 号:TB332[一般工业技术—材料科学与工程]

 

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