Unraveling the microbial contamination characteristics of pork farms and disinfection efficacy assessment via high-throughput sequencing  

作　　者：Feng Liu Jiele Ma Lichang Sui Wen Wang Yingping Xiao Qingli Dong Xingning Xiao 

机构地区：[1]College of Health Science and Engineering,University of Shanghai for Science and Technology,516 Jungong Road,Shanghai 200093,China [2]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,MOA Laboratory of Quality&Safety Risk Assessment for Agro-Products(Hangzhou),Institute of Agro-Product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China [3]Key Laboratory of Zoonosis Prevention and Control of Guangdong Province,College of Veterinary Medicine,South China Agricultural University,Guangzhou 510640,China [4]College of Life Science,China Jiliang University,Hangzhou 310018,China

出　　处：《Animal Diseases》2025年第1期101-112,共12页动物疾病(英文)

基　　金：supported by the Natural Science Foundation of China(32302233,32472466);the Natural Science Foundation of Zhejiang(LZ24C200004);the Key Research and Development Program of Zhejiang Province(2022C02049);the Key Research and Development Program of Ningbo(2022Z178);the Ministry of Agriculture and Rural Affairs(20244027);the WalMart Foundation(UA2020-152,UA2021-247);the State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(2021DG700024-KF202517);the Zhejiang Provincial Department of Agriculture and Rural Affairs Project(2024SNJF044);the Key Research and Development Program of Zhuji(2022J10).

摘　　要：The contamination of pork during breeding can negatively impact both food quality and safety.Sodium dichloroiso-cyanurate(NaDCC),a chlorine-containing disinfectant,is widely used in animal environments.In this study,16S rRNA sequencing was used to determine the bacterial communities in a pork farm.We also assessed the effectiveness of NaDCC disinfection by analyzing microbial diversity.The results revealed that the predominant genera in pork skin samples and environmental samples were Staphylococcus and Acinetobacter,respectively.Escherichia coli and Strep-tococcus equinus were present in all the samples,suggesting that NaDCC is not fully effective in preventing zoonotic pathogen contamination.The Chao1 and Shannon indices for sow skins increased after disinfection.No significant differences were observed in the microbiological composition of sow barn samples before and after disinfection(p>0.1).Additionally,bacterial abundance in sow skin was strongly correlated with abundance on pen floors(r=0.906,p<0.05),indicating potential cross-contamination between these sample types.Conversely,the bacterial abun-dance in the floor samples was weakly correlated with that in the other samples,suggesting that NaDCC is effective as a decontaminant.This study provides valuable insights into microbial contamination on pork farms and under-scores the importance of proper disinfection practices.This study also offers scientific recommendations for improv-ing disinfection protocols.

关 键 词：Pork farm NaDCC disinfection Bacterial contamination One health 

分 类 号：Q93[生物学—微生物学]