Isolation of four carbapenem-resistant gram-negative species from a single fly  

作　　者：Hanyu Wang Hongwei Zhou Gongxiang Chen Ning Dong 

机构地区：[1]Department of Clinical Laboratory Medicine,Second Affiliated Hospital of Zhejiang University,Hangzhou,China [2]Department of Medical Microbiology,School of Biology and Basic Medical Sciences,Medical College,Soochow University,Suzhou,China [3]MOE Key Laboratory of Geriatric Diseases and Immunology,Suzhou Key Laboratory of Pathogen Bioscience and Anti-infective Medicine,School of Biology&Basic Medical Sciences,Suzhou Medical College,Soochow University,Suzhou,China [4]Center for Clinical Big Data and Analytics,The Second Affiliated Hospital and School of Public Health,Zhejiang University School of Medicine,Hangzhou,Zhejiang,China

出　　处：《Animal Diseases》2025年第1期124-128,共5页动物疾病(英文)

基　　金：funded by the National Natural Science Foundation of China(Grant No.32300156);the Natural Science Foundation of Jiangsu Province(Grant No.BK20220493).

摘　　要：The widespread occurrence of carbapenem-resistant organisms has garnered significant public attention.Arthro-pods,including flies,are important vectors of multidrug-resistant bacteria.In this study,we reported the simultane-ous carriage of four carbapenem-resistant isolates from different species,namely,Escherichia coli(E.coli),Providencia manganoxydans(P.manganoxydan),Myroides odoratimimus(M.odoratimimus)and Proteus mirabilis(P.mirabilis),from a single fly in China.These isolates were characterized through antimicrobial susceptibility testing,conjuga-tion assays,whole-genome sequencing,and bioinformatics analysis.M.odoratimimus showed intrinsic resistance to carbapenems.The mechanisms of carbapenem resistance in E.coli,P.manganoxydans,and P.mirabilis were due to the production of NDM-5,NDM-1 and NDM-1,respectively.Genetic context of the bla_(NDM) genes in these three isolates varied.The bla_(NDM-5) gene in E.coli was located on an IncHI2/HI2A multidrug-resistant plasmid,which was con-jugatively transferable.The bla_(NDM-1) gene in P.mirabilis resided on the pPM14-NDM_123k-like nonconjugative plasmid.The bla_(NDM-1) gene in P.manganoxydans was found in a nonconjugatively transferable,multidrug-resistant region.The results of this study enhance our understanding of the dissemination of carbapenem-resistant organisms and sug-gest the need for a more comprehensive approach to antibiotic resistance research encompassing humans,animals,and the environment.

关 键 词：FLY Carbapenem-resistant organism Whole-genome sequencing Phenotypic characterization blaNDM 

分 类 号：R44[医药卫生—诊断学]