择时针刺对神经病理性疼痛小鼠TRPV1和SP表达的不同调整作用  

Different Effects of Acupuncture at Different Time on the Expression of TRPV1 and SP in Neuropathic Pain Mice

作　　者：曾灵活 蒋涵 蔡定均[1] ZENG Linghuo;JIANG Han;CAI Dingjun(Acupuncture and Moxibustion College,Chengdu University of TCM,Chengdu 611137,China)

机构地区：[1]成都中医药大学针灸推拿学院,成都611137

出　　处：《世界科学技术-中医药现代化》2025年第2期592-600,共9页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：四川省科技计划项目(2021YJ0177):基于SP-Mrgprb2R研究择时针刺治疗神经病理性疼痛的钟控神经-免疫调节机制,负责人:赵征宇。

摘　　要：目的观察择时针刺对神经病理性疼痛(Neuropathic pain,NP)小鼠背根神经节(Dorsal root ganglion,DRG)中香草酸瞬时受体亚型1(Transient receptor potential vanilloid 1,TRPV1)与P物质(Substance P,SP)的不同调整作用。方法将64只C57BL/6J小鼠随机分为两个干预组,分别为ZT8和ZT16组,其中两个干预组中又随机分为空白组(8只)、假手术组(8只)、模型组(8只)和针刺组(8只)。模型组和针刺组采用坐骨神经结扎法制备慢性压迫性损伤(Chronic constriction injury,CCI)模型,假手术组仅暴露坐骨神经后缝合伤口。于造模后第8天开始对针刺组小鼠进行针刺治疗,两个组干预方法相同,仅针刺时间不同;穴取手术侧“阳陵泉”,手动针刺、行针,每天1次,每次15 min,连续干预7天后取材。期间分别于造模后第3、7、10、14天测试各组小鼠的足底机械痛和热痛阈值以评定其疼痛情况。采用免疫荧光双标染色法观察小鼠手术侧L4-6 DRG内SP和TRPV1的表达情况,采用酶联免疫吸附试验(ELISA)测定小鼠患侧坐骨神经周围组织及足背腓侧皮肤的SP含量。结果(1)针刺干预可改善CCI小鼠的机械和热痛觉过敏;且ZT8时间点针刺效果优于ZT16时间点。(2)针刺可显著降低CCI小鼠DRG内TRPV1和SP的表达,且ZT8时间点的针刺效果优于ZT16时间点。(3)针刺可显著降低CCI小鼠损伤神经局部周围组织SP的浓度,且ZT8时间点的针刺效果优于ZT16时间点。(4)针刺可显著降低CCI小鼠损伤神经远端皮肤SP的浓度,且ZT8、ZT16之间无明显差异。结论择时针刺对CCI小鼠痛觉过敏有不同的调节作用,ZT8时间点的调节作用优于ZT16时间点,可能与择时针刺对CCI小鼠DRG内TRPV1和SP表达及损伤神经局部SP浓度的不同调节作用相关。Objective To observe the effect of acupuncture at different time on dorsal root ganglion(DRG)in neuropathic pain(NP)mice.Different regulatory effects of transient receptor potential vanilloid 1(TRPV1)and substance P(SP)in DRG.Methods A total of64 C57BL/6J mice were randomly divided into two intervention groups:ZT8 group and ZT16 group,among which two intervention groups were randomly divided into control group(8 mice),sham-operation group(8 mice),model group(8 mice)and acupuncture group(8 mice).The chronic constriction injury(CCI)model was prepared by sciatic nerve ligation in model group and acupuncture group,and only the sutured wound after sciatic nerve was exposed in sham-operation group.On the 8th day after operation,acupuncture treatment was performed on mice in the acupuncture group.The intervention methods were the same in the two groups,only the acupuncture time was different.The"Yanglingquan"on the surgical side was taken at the acupoint,and manual acupuncture and needle were performed once a day for 15 minutes each time.The mechanical pain and thermal pain thresholds of each group of mice were measured at 3,7,10 and 14 days after modeling to evaluate their pain.The expression of SP and TRPV1 in the L4-6 DRG on the surgical side of mice was observed by immunofluorescence double labeling method,and the SP content in the tissue around the sciatic nerve on the affected side and the skin on the dorsal fibra of the foot was determined by enzymed-linked immunosorbent assay(ELISA).Results①Acupuncture intervention could improve mechanical and thermal hyperalgesia in CCI mice.And ZT8 time point acupuncture effect is better than ZT16 time point.②Acupuncture can significantly reduce the expression of TRPV1 and SP in DRG of CCI mice,and the effect of acupuncture at ZT8 time point is better than that at ZT16 time point.③Acupuncture can significantly reduce the SP concentration in the peripheral tissue of CCI mice,and the effect of acupuncture at ZT8 time point is better than that at ZT16 time point.④Acupunct

关 键 词：择时针刺 神经病理性疼痛 TRPV1 P物质 

分 类 号：R245.31[医药卫生—针灸推拿学]