144份番茄种质资源的抗病性检测与评价  

Detection and evaluation of disease resistance in 144 tomato germplasm resources

作　　者：权建华 刘玉玲 许可红 祁世明 鄂利锋 QUAN Jianhua;LIU Yuling;XU Kehong;QI Shiming;E Lifeng(College of Agriculture and Ecological Engineering,Hexi University,Zhangye 734000,China)

机构地区：[1]河西学院农业与生态工程学院,甘肃张掖734000

出　　处：《甘肃农业大学学报》2025年第1期120-127,共8页Journal of Gansu Agricultural University

基　　金：甘肃省陇原青年创新创业人才项目(2021LQGR17);甘肃省高等学校创新基金项目(2022A-117)。

摘　　要：【目的】科学分析和评价不同材料的多抗性,为番茄育种提供更多优质的抗病种质资源。【方法】利用目前广泛应用的Ty-1、I-2、Ph-3、Cf-9、Mi-1等5个抗病基因分子标记,对144份番茄种质资源进行抗病性检测和室内接种鉴定。【结果】在检测的144份材料中,只含有1种抗病基因的材料有22份,占15%,含有2种抗病基因的材料有76份,占53%,含有3种抗病基因的材料有38份,占26%,含有4种抗病基因的材料有8份,占6%,没有检测到同时含有5种抗病基因的材料,含有Ty-1基因的材料有88份,占61%,含有I-2基因的材料有63份,占44%,含有Ph-3基因的材料有48份,占33%,含有Cf-9基因的材料有68份,占47%,含有Mi-1基因的材料有53份,占37%。【结论】含有2种抗病基因的材料最多,含有4种抗病基因的材料最少。对含有4种抗病基因的材料进行室内接种鉴定,其抗病水平均达到抗病(R)及以上,将分子标记检测技术和室内接种鉴定相结合,可快速准确选育番茄多抗性育种资源。【Objective】To analyze and evaluate the multi-resistance of different materials in order to provide more high quality germplasm resources for tomato breeding.【Method】144 tomato germplasm re⁃sources were tested and inoculated in the laboratory using five molecular markers of disease resistance genes,namely Ty-1,I-2,Ph-3,Cf-9 and Mi-1,which are currently widely used.【Result】Of the 144 mate⁃rials tested,22 materials contained only one type of resistance gene,accounting for 15%;76 materials con⁃tained two types of resistance genes,accounting for 53%;38 materials contained three types of resistance genes,accounting for 26%;8 materials contained four types of resistance genes,accounting for 6%;no mate⁃rials containing five resistance genes at the same time were detected,including 88 materials containing Ty-1 gene,accounting for 61%;63 materials containing I-2 gene,accounting for 44%;48 materials containing Ph-3 gene,accounting for 33%;68 materials containing Cf-9 gene,accounting for 47%.There were 53 materials containing Mi-1 gene,accounting for 37%.【Conclusion】The materials containing 2 resistance genes were the most,and the materials containing 4 resistance genes were the least.The materials contain⁃ing 4 kinds of resistance genes were identified by indoor inoculation,and their resistance levels were all up to(R)or above.The combination of molecular marker detection technology and indoor inoculation identifi⁃cation can quickly and accurately select tomato multi-resistance breeding resources.

关 键 词：番茄 育种 种质资源 抗病 

分 类 号：S641.2[农业科学—蔬菜学]