系统性红斑狼疮肾炎患者血清LRG1和Thiol水平表达及临床价值研究  

Study on the Expression and Clinical Value of Serum LRG1 and Thiol Levels in Patients with Systemic Lupus Erythematosus Nephritis

作　　者：胡鹤鸣[1] 朱现菊 钟艳艳 王虹[2] HU Heming;ZHU Xianju;ZHONG Yanyan;WANG Hong(Department of Clinical Laboratory,Qingdao Eighth People’s Hospital,Shandong Qingdao 266041,China;Department of Nephrology,Qingdao Eighth People’s Hospital,Shandong Qingdao 266041,China;Department of Rheumatology and Immunology,Affiliated Hospital of Qingdao University,Qingdao Shandong 266700,China)

机构地区：[1]青岛市第八人民医院检验科,山东青岛266041 [2]青岛市第八人民医院肾内科,山东青岛266041 [3]青岛大学附属医院风湿免疫科,山东青岛266700

出　　处：《现代检验医学杂志》2025年第2期114-118,共5页Journal of Modern Laboratory Medicine

基　　金：山东省医药卫生科技发展计划项目(202014050420)。

摘　　要：目的探讨系统性红斑狼疮肾炎(SLEN)患者血清富亮氨酸α-2糖蛋白-1(LRG1)、硫醇(Thiol)水平表达与自身免疫抗体及肾功能损伤的相关性。方法选取2021年1月~2023年12月青岛市第八人民医院肾内科和青岛大学附属医院风湿免疫科收治的SLEN患者80例(SLEN组)和单纯SLE患者80例(非SLEN组)。酶联免疫吸附法(ELISA)检测血清LRG1水平,比色法检测血清Thiols水平。采用Pearson/Spearman相关性分析SLEN患者血清LRG1,Thiol与自身免疫抗体及肾功能指标的相关性;受试者工作特征(ROC)曲线分析血清LRG1,Thiol水平对SLEN的诊断价值。结果SLEN组血清LRG1(26.00±8.76μg/ml),Scr(98.03±49.71μmol/L)高于非SLEN组(16.71±5.51μg/ml,73.85±33.51μmol/L),Thiol(80.69±36.90μmol/L)低于非SLEN组(131.49±45.34μmol/L),差异具有统计学意义(t=8.026,3.608,7.772,均P<0.001)。Pearson/Spearman相关性分析SLEN患者血清LRG1与抗双链-DNA(dsDNA)抗体阳性、抗补体1q(C1q)抗体阳性、抗Smith抗体阳性、血肌酐(Scr)、24h尿蛋白呈正相关(r=0.226~0.637,均P<0.05),与估计的肾小球滤过率(eGFR)呈负相关(r=-0.633,P<0.05);血清Thiol与抗dsDNA抗体阳性、抗C1q抗体阳性、抗Smith抗体阳性、Scr,24h尿蛋白呈负相关(r=-0.612~-0.256,均P<0.05),与eGFR呈正相关(r=0.614,P<0.05)。血清LRG1联合Thiol诊断SLEN的AUC(95%CI)为0.907(0.851~0.947),大于血清LRG1,Thiol单独诊断的0.802(0.731~0.860),0.808(0.738~0.866),差异具有统计学意义(Z=3.450,3.749,均P<0.05)。结论SLEN患者血清LRG1水平升高、Thiol水平降低与自身免疫抗体阳性表达及肾功能损伤密切相关,联合检测血清LRG1和Thiols可为诊断SLEN提供参考。Objective To investigate the correlation of serum leucine-rich alpha-2 glycoprotein-1(LRG1)and Thiol levels with autoimmune antibodies and renal injury in patients with systemic lupus erythematosus nephritis(SLEN).Methods 80 SLEN patients(SLEN group)and 80 simple SLE patients(non-SLEN group)admitted to the Department of Nephrology at Qingdao Eighth People’s Hospital and the Department of Rheumatology and Immunology at Affiliated Hospital of Qingdao University from January 2021 to December 2023 were selected.An enzyme-linked immunosorbent assay(ELISA)was used to detect serum LRG1 levels,while a colorimetric assay was used to detect serum Thiol levels.Pearson/Spearman correlation analysis was used to investigate the correlation between serum LRG1 and Thiol levels,autoimmune antibodies,and renal function indicators in SLEN patients.The diagnostic value of serum LRG1 and Thiol levels in SLEN was analyzed using receiver characteristic curves.Results The serum LRG1(26.00±8.76μg/ml)and Scr(98.03±49.71μmol/L)in the SLEN group were higher than those in the non-SLEN group(16.71±5.51μg/ml,73.85±33.51μmol/L),and Thiol(80.69±36.90μmol/L)was lower than those in the non-SLEN group(131.49±45.34μmol/L),the differences were statistically significant(t=8.026,3.608,7.772,all P<0.001).Pearson/Spearman correlation analysis showed that serum LRG1 in SLEN patients was positively correlated with antidouble-stranded deoxyribonucleic acid(dsDNA)antibody,anti-complement 1q(C1q)antibody,anti-Smith antibody,serum creatinine(Scr),24-hour urine protein(r=0.226~0.637,all P<0.05),and negatively correlated with estimated glomerular filtration rate(eGFR)(r=-0.633,P<0.05).Serum Thiol was negatively correlated with anti-dsDNA antibodies,anti C1q antibodies,anti-Smith antibodies,Scr and 24-hour urine protein(r=-0.612~-0.256,all P<0.05),and positively correlated with eGFR(r=0.614,P<0.05).The AUC(95%CI)of serum LRG1 combined with Thiol levels for diagnosing of SLEN was 0.907(0.851~0.947),which was higher than that of serum LRG1 and Thiol a

关 键 词：系统性红斑狼疮肾炎 富亮氨酸α-2糖蛋白-1 硫醇 自身免疫抗体 肾功能损伤 

分 类 号：R593.242[医药卫生—内科学] R392.11[医药卫生—临床医学]