老年2型糖尿病患者血清USP14和GRB2水平与下肢血管病变的相关性研究  

Study on the Correlation between Serum USP14 and GRB2 Levels and Vascular Lesions of the Lower Limbs in Elderly Patients with T2DM

作　　者：李丽华 史春艳 张欢[1] 柴雪妍 LI Lihua;SHI Chunyan;ZHANG Huan;CHAI Xueyan(Department of Endocrinology,Beijing Nuclear Industry Hospital,Beijing 102413,China;Department of Clinical Laboratory,Beijing Nuclear Industry Hospital,Beijing 102413,China)

机构地区：[1]北京核工业医院内分泌科,北京102413 [2]北京核工业医院检验科,北京102413

出　　处：《现代检验医学杂志》2025年第2期140-144,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探讨老年2型糖尿病(T2DM)患者血清泛素特异性蛋白酶14(USP14)和生长因子受体结合蛋白2(GRB2)水平与下肢血管病变(LEAD)的相关性。方法选取北京核工业医院2020年7月~2023年8月收治的老年T2DM患者153例,根据有无LEAD分为病变组(n=64)和非病变组(n=89);另选取同期与老年T2DM患者年龄相匹配的153例健康体检志愿者作为对照组。采用酶联免疫吸附法(ELISA)检测患者血清USP14和GRB2水平。多因素Logistic回归分析影响T2DM患者LEAD发生的因素;受试者工作特征曲线(ROC)分析老年T2DM患者USP14,GRB2水平对发生LEAD的诊断价值。结果与对照组相比,病变组和非病变组中血清USP14(8.56±1.57ng/ml,6.34±1.27ng/ml vs 4.23±1.02ng/ml),GRB2水平(7.45±1.56ng/ml,5.23±1.12ng/ml vs 3.19±0.78ng/ml)明显增加,差异具有统计学意义(q=33.555,18.259;37.326,19.960,均P<0.05)。A级、B级和C级血清USP14(7.14±1.31ng/ml,8.67±1.52ng/ml,9.98±2.01ng/ml),GRB2(6.31±1.25ng/ml,7.47±1.58ng/ml,8.69±1.74ng/ml)水平依次增加,差异具有统计学意义(F=14.754,11.404,均P<0.05)。多因素Logistic回归分析结果显示,USP14,GRB2,TC,TG,LDL-C和HbA1c均是影响老年T2DM患者LEAD发生的影响因素(Wald χ^(2)=11.618~41.458,均P<0.05);ROC曲线结果显示,血清USP14和GRB2联合诊断LEAD的曲线下面积(AUC)高于USP14和GRB2单独诊断(Z=2.706,3.124,均P<0.05)。结论USP14和GRB2在老年T2DM并发LEAD患者血清中均呈高表达水平,随LEAD分级增加而升高,可作为诊断老年T2DM并发LEAD的血清标志物,两者联合检测效能更佳。Objective To investigate the correlation between serum ubiquitin-specific protease 14(USP14)and growth factor receptor binding protein 2(GRB2)levels and vascular lesions of the lower limbs(LEAD)in elderly patients with type 2 diabetes(T2DM).Methods 153 elderly T2DM patients admitted to Beijing Nuclear Industrial Hospital from July 2020 to August 2023 were selected and divided into lesion group(n=64)and non-lesion group(n=89)according to the presence or absence of LEAD,in addition and 153 healthy volunteers who matched the age of elderly T2DM patients in the same period were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum USP14 and GRB2 levels in patients.Multifactorial Logistic regression was used to analyze the factors affecting the occurrence of LEAD in patients with elderly T2DM.Receiver operating curve(ROC)analysis of USP14 and GRB2 levels in elderly T2DM patients for the diagnostic value of LEAD occurrence.Results Compared with the control group,serum USP14(8.56±1.57ng/ml,6.34±1.27ng/ml vs 4.23±1.02ng/ml),GRB2 levels(7.45±1.56ng/ml,5.23±1.12ng/ml vs 3.19±0.78ng/ml)were significantly increased in the diseased and non-diseased groups,and the differences were statistically significant(q=33.555,18.259;37.326,19.960,all P<0.05).Class A,B and C serum USP14(7.14±1.31ng/ml,8.67±1.52ng/ml,9.98±2.01ng/ml),GRB2 levels(6.31±1.25ng/ml,7.47±1.58ng/ml,8.69±1.74ng/ml)increased in that order,and the differences were all statistically significant(F=14.754,11.404,all P<0.05).Multifactorial Logistic regression analysis showed that USP14,GRB2,TC,TG,LDL-C and HbA1c were all influencing factors affecting the occurrence of LEAD in patients with T2DM(Wald χ^(2)=11.618~41.458,all P<0.05).The results of the ROC curve showed that the area under the curve(AUC)of the combined diagnosis of LEAD by serum USP14 and GRB2 was greater than that of USP14 and GRB2 diagnosed alone(Z=2.706,3.124,all P<0.05).Conclusion USP14 and GRB2 were both highly expressed in the serum of patients with

关 键 词：2型糖尿病 泛素特异性蛋白酶14 生长因子受体结合蛋白2 下肢血管病变 

分 类 号：R587.1[医药卫生—内分泌] R392.11[医药卫生—内科学]