支气管哮喘儿童血清lncRNA KCNQ1OT1和FGF2水平表达对病情控制水平评估的价值  

Value of Serum lncRNA KCNQ1OT1 and FGF2 Levels in the Assessment of Disease Control in Children with Bronchial Asthma

作　　者：訾和平 杨晓荣 高貂艳 ZI Heping;YANG Xiaorong;GAO Diaoyan(Department of Pediatrics,Yulin Hospital of Traditional Chinese Medicine,Shaanxi Yulin 719000,China)

机构地区：[1]榆林市中医医院儿科,陕西榆林719000

出　　处：《现代检验医学杂志》2025年第2期169-173,185,共6页Journal of Modern Laboratory Medicine

基　　金：陕西省卫生健康科研项目(2021D099)。

摘　　要：目的探讨血清长链非编码RNA(lncRNA)钾电压门控通道亚家族Q成员1反向转录物1(KCNQ1OT1)、成纤维细胞生长因子2(FGF2)在小儿支气管哮喘病情和控制水平评估中的价值。方法选择2021年3月~2023年3月榆林市中医医院收治的96例支气管哮喘患儿(哮喘组)和76例健康儿童(对照组)。根据支气管哮喘病情将患儿分为轻度组(n=54)和重度组(n=42);根据治疗后疾病控制情况将患儿分为哮喘控制组(n=61)和哮喘未控制组(n=35)。检测各组血清lncRNA KCNQ1OT1和FGF2水平,多因素Logistic回归分析影响支气管哮喘疾病控制的因素;受试者工作特征(ROC)曲线评估lncRNA KCNQ1OT1和FGF2预测支气管哮喘疾病控制的效能。结果哮喘组血清lncRNA KCNQ1OT1(2.35±0.71),FGF2(27.35±6.29 pg/ml)水平均高于对照组(1.51±0.33,15.32±4.08 pg/ml),差异具有统计学意义(t=9.527,14.437,均P<0.001)。重度组血清lncRNA KCNQ1OT1(2.59±0.35),FGF2(28.65±1.03 pg/ml)水平高于轻度组(2.16±0.41,26.34±1.24 pg/ml),差异具有统计学意义(t=5.429,9.737,均P<0.001)。哮喘未控制组血清lncRNA KCNQ1OT1(2.63±0.27),FGF2(29.02±1.41 pg/ml)高于哮喘控制组(2.19±0.35,26.39±1.52 pg/ml),差异具有统计学意义(t=6.417,8.374,均P<0.001)。高lncRNA KCNQ1OT1和FGF2水平是支气管哮喘患儿疾病未控制的危险因素(Wald χ^(2)=8.792,5.638,均P<0.05)。lncRNA KCNQ1OT1,FGF2联合预测支气管哮喘患儿疾病未控制的AUC(95%CI)高于单独预测,差异具有统计学意义(Z=3.250,2.096,均P<0.05)。结论支气管哮喘患儿血清lncRNA KCNQ1OT1和FGF2均增高,与支气管哮喘病情加重和疾病未控制有关,联合lncRNA KCNQ1OT1和FGF2可预测支气管哮喘疾病控制水平。Objective To explore the value of serum long non-coding RNA KCNQl opioid strand transcript 1(lncRNA KCNQ1OT1)and fibroblast growth factor 2(FGF2)in the evaluation of the condition and control level of pediatric bronchial asthma.Methods 96 children with bronchial asthma(Asthma group)and 76 healthy children(Control group)admitted to Yulin Hospital of Traditional Chinese Medicine from March 2021 to March 2023 were selected.According to the severity of bronchial asthma,the children were divided into a mild group(n=54)and a severe group(n=42).According to the disease control status after treatment,the children were divided into an asthma Control group(n=61)and an uncontrolled Asthma group(n=35).The serum levels of lncRNA KCNQ1OT1 and FGF2 were detected in each group.Multivariate Logistic regression analysis was performed to identify the factors affecting bronchial asthma disease control.The performance of lncRNA KCNQ1OT1 and FGF2 in predicting the control of bronchial asthma disease was evaluated using the receiver operating characteristic curve.Results The serum levels of lncRNA KCNQ1OT1(2.35±0.71)and FGF2(27.35±6.29 pg/ml)in the Asthma group were higher than those in the Control group(1.51±0.33,15.32±4.08 pg/ml),and the differences were statistically significant(t=9.527,14.437,all P<0.001).The serum levels of lncRNA KCNQ1OT1(2.59±0.35)and FGF2(28.65±1.03 pg/ml)in the severe group were higher than those in the mild group(2.16±0.41,26.34±1.24 pg/ml),and the differences were statistically significant(t=5.429,9.737,all P<0.001).The serum levels of lncRNA KCNQ1OT1(2.63±0.27)and FGF2(29.02±1.41 pg/ml)in the uncontrolled asthma group were higher than those in the controlled asthma group(2.19±0.35,26.39±1.52 pg/ml),and the differences were statistically significant(t=5.417,8.374,all P<0.001).High lncRNA KCNQ1OT1 and FGF2 levels were risk factors for uncontrolled disease in children with bronchial asthma(all P<0.05).The AUC of lncRNA KCNQ1OT1 and FGF2 combined to predict uncontrolled disease in children with bron

关 键 词：支气管哮喘 非编码RNA 钾电压门控通道亚家族Q成员1反向转录物1 成纤维细胞生长因子2 

分 类 号：R562.25[医药卫生—呼吸系统] R392.11[医药卫生—内科学]