退化灵芝菌株的纯化与复壮技术研究  

Study on Purification and Rejuvenation Technologyof Degenerated Ganoderma lingzhi Strains

作　　者：李红[1] 刘岩岩[1] 刘俊杰[1] 赵玉梅 LI Hong;LIU Yanyan;LIU Junjie;ZHAO Yumei(Edible Fungi Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China;Center of Advanced Equipment Manufacturing Industry Development Promotion in Fushun City,Fushun 113000,China)

机构地区：[1]辽宁省农业科学院食用菌研究所,辽宁沈阳110161 [2]抚顺市先进装备制造业发展促进中心,辽宁抚顺113000

出　　处：《中国食用菌》2025年第1期20-25,共6页Edible Fungi of China

基　　金：辽宁省新宾县食用菌科技特派团资助项目(2023JH5/10400122);抚顺市科技共建项目(2021—2023);辽宁省食用菌首席科技特派员项目(2024JH/10400163)。

摘　　要：采用高温-菌丝尖端处理技术对1株退化的灵芝菌株进行纯化和复壮,通过菌丝生长情况、菌丝的显微形态、菌丝中漆酶和羧甲基纤维素酶活性、栽培后子实体的农艺性状等指标评价复壮效果。结果表明,复壮后菌株生长情况良好,菌丝无色透明、有横膈膜、粗细不均、锁状联合明显、尖端呈放射性分支,菌管表面光滑无纹饰、管间清晰无杂质。复壮菌株菌丝生长速度是原代菌株的1.46倍;与原代菌株相比漆酶活性提高了60%,羧甲基纤维素酶活性提高了85%。栽培试验表明复壮菌株子实体的农艺性状优于原代菌株,菌包污染率下降了25%,生物学效率提高了3.38%。Purification and rejuvenation of a degenerating strain of Ganoderma lingzhi was carried out by the technique of high temperature and hyphal tip treatment.The effect of rejuvenation was evaluated by the growth of mycelia,the microscopic morphology of mycelia,the activities of laccase and carboxymethyl cellulase in mycelia,and the agronomic traits of fruit bodies after cultivation.The results showed that the strain grew well after rejuvenation,the hyphal was colorless and transparent,with diaphragms,uneven thickness,obvious clamp connection,radioactive branches at the tip,the surface of the tube is smooth and no decoration,and the intertube was clear and inclusion-free.The mycelial growth rate of the rejuvenated strain was 1.46 times that of the original strain.The enzymes activities of laccase and carboxymethyl cellulase increased by 60%and 85%respectively.Cultivation test shows that all the agronomic characters of fruit bodies were better than those of the original strain,and pollution rate decreased by 25%,biological efficiency increased by 3.38%.

关 键 词：灵芝 退化 复壮 栽培 

分 类 号：S646.2[农业科学—蔬菜学]