镉污染下丛枝菌根真菌和纳米二氧化钛对萱草光合生理和镉吸收的影响  

Effects of arbuscular mycorrhizal fungi and TiO2 nanoparticles on the photosynthetic physiology and cadmium uptake of Hemerocallis fulva under cadmium pollution

作　　者：刘振羽 郝建朝 张杰西 郝婷婷 陈晖 刘家宁 苑红丹 LIU Zhenyu;HAO Jianchao;ZHANG Jiexi;HAO Tingting;CHEN Hui;LIU Jianing;YUAN Hongdan(Tianjin Eco-Environmental Monitoring Center,Tianjin 300191,China;College of Agronomy&Resources and Environment,Tianjin Agricultural University,Tianjin 300392,China;Beijing Construction Engineering Group Environmental Remediation Co.Ltd.,Beijing 100015,China;Tianjin Tianbin Tongsheng Environmental Technology Co.Ltd.,Tianjin 300110,China;College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193,China)

机构地区：[1]天津市生态环境监测中心,天津300191 [2]天津农学院农学与资源环境学院,天津300392 [3]北京建工环境修复股份有限公司,北京100015 [4]天津天滨同盛环境科技有限公司,天津300110 [5]中国农业大学资源与环境学院,北京100193

出　　处：《草业科学》2025年第3期638-650,共13页Pratacultural Science

基　　金：国家重点研发计划项目(2023YFC3709105)。

摘　　要：研究微生物和纳米材料共同对重金属污染土壤的修复效应对重金属污染防治具有重要意义,以萱草-1(Hemerocallis fulva)‘Golden Doll’为试材,采用室内盆栽试验,探究50 mg·kg镉(Cd)污染下接种丛枝菌根真菌(AMF)与根施T_(1)(100 mg·L^(-1))、T_(2)(200 mg·L^(-1))、T3(400 mg·L^(-1))浓度纳米二氧化钛(TiO_(2) NPs)及其复合处理对萱草生长、叶绿素和叶绿素荧光参数、气体交换参数、Cd吸收及抗氧化系统的影响,以探明两者在Cd污染下提高萱草光合生理以及促进Cd吸收的作用。结果发现:Cd污染土壤种植萱草的株高、叶片数、地上部和根系生物量均显著下降,根施不同浓度TiO_(2) NPs和TiO_(2) NPs+AMF处理均可以缓解萱草生长受到的抑制。与Cd处理相比,Cd+T_(2)+AMF处理的萱草叶绿素a、叶绿素b、叶绿素a+b、叶绿素a/b以及类胡萝卜含量分别增加了53.8%、29.7%、48.6%、18.4%和25.8%,叶绿素荧光参数中PSⅡ最大光化学效率(F_(v)/F_(m))、PSⅡ潜在活性(F_(v)/F_(o))和光化学猝灭系数(qP)分别增加14.6%、60.7%和67.6%,而非光化学猝灭系数(NPQ)呈下降趋势。Cd污染抑制了萱草净光合速率(P_(n))、蒸腾速率(T_(r))和气孔导度(G_(s))的增长,Cd+T_(2)+AMF处理能显著提高P_(n)。同时,根施不同浓度TiO_(2) NPs和TiO_(2) NPs+AMF处理能够降低萱草地上部和根系Cd含量,提高萱草Cd转运系数并降低富集系数,激活植物叶片过氧化物酶(APX)、过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性,并增加非酶抗氧化剂抗坏血酸(AsA)、谷胱甘肽(GSH)以及总黄酮(TF)含量来缓解萱草受到的氧化胁迫。总体上,Cd+T_(2)+AMF处理增强萱草抗Cd污染胁迫的效果最好。Studying the combined remediation effects the combined remediation effects of microorganisms soil is of considerable significance for the prevention and control of heavy metal pollution.In this study,we conducted an-1 indoor pot experiment to investigate the effects of 50 mg·kg cadmium(Cd)pollution on the growth,chlorophyll content,chlorophyll fluorescence and gas exchange parameters,Cd uptake using Hemerocallis fulva(Golden Doll).Additionally,we also investigated the antioxidant system of H.fulva treated with an inoculation of arbuscular mycorrhizal fungi(AMF)and root application of T_(1)(100 mg·L^(-1)),T_(2)(200 mg·L^(-1)),T3(400 mg·L^(-1))concentrations of titanium dioxide nanoparticles(TiO_(2) NPs),as both independent and combined treatments.The aim of the study was to establish the effects of these two treatments on improving the photosynthetic physiology of H.fulva and promoting Cd uptake in Cd-contaminated soils.The results revealed that compared with the control(CK)treatment,there were significant reductions in the height,number of leaves,and aboveground and root biomasses of H.fulva under Cd pollution.Root application of different concentrations of TiO_(2) NPs and the combined TiO_(2) NPs+AMF treatment were found to alleviate the Cd-induced growth inhibition of H.fulva.Compared with the Cd treatment,the Cd+T_(2)+AMF treatment increased chlorophyll a,chlorophyll b,chlorophyll a+b,chlorophyll a/b,and carotenoid contents of H.fulva by 53.8%,29.7%,48.6%,18.4%,and 25.8%,respectively.Among the assessed chlorophyll fluorescence parameters,the maximum photochemical efficiency(F_(v)/F_(m)),potential activity(F_(v)/F_(o)),and photochemical quenching coefficient(qP)of photosystem II were increased by 14.6%,60.7%,and 67.6%,respectively,whereas the non-photochemical quenching coefficient(NPQ)showed a declining trend.Cd pollution inhibited the net photosynthetic rate(P_(n)),transpiration rate,and stomatal conductance of H.fulva,whereas the Cd+T_(2)+AMF treatment restored the value of P_(n) to a level comparable to

关 键 词：丛枝菌根真菌 重金属 纳米二氧化钛 萱草 光合特性 叶绿素 抗氧化酶 

分 类 号：S68[农业科学—观赏园艺]