藤黄健骨胶囊激活Wnt5a/β-catenin信号轴调控卵巢摘除模型鼠的骨代谢  

Tenghuang Jiangu Capsule regulates bone metabolism by activation of Wnt5a/β-catenin signaling axis in postmenopausal osteoporosis rats

作　　者：颜春鲁[1] 肖小龙 宋佳眙 王玉洁 张捷 常伟荣 安方玉[1] 袁万英 王芳[1] 赵亚娜 耿广琴[1] YAN Chunlu;XIAO Xiaolong;SONG Jiayi;WANG Yujie;ZHANG Jie;CHANG Weirong;AN Fangyu;YUAN Wanying;WANG Fang;ZHAO Yana;GENG Guangqin(Gansu University of Chinese Medicine,Lanzhou 730000,China;TCM hospital of Dingxi,Dingxi 743099,China;Tianshui health school,Tianshui 741000,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]定西市中医医院,甘肃定西743099 [3]天水市卫生学校,甘肃天水741000

出　　处：《中国骨质疏松杂志》2025年第3期335-342,402,共9页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金项目(82060872);甘肃省中医药管理局科研项目(GZKG-2024-88);高校教师创新基金项目(2024B-096,2024A-089);甘肃中医药大学教学研究与改革项目(ZHXM-202307);定西市科技计划项目(DX2024BY018)。

摘　　要：目的探究藤黄健骨胶囊(Tenghuang Jiangu capsule,TJC)激活Wnt5a/β-catenin信号轴对卵巢摘除模型(ovariectomy,OVX)大鼠骨代谢的的影响。方法60只SPF级SD大鼠,分为6组,每组10只,分组如下:假手术组(SO组)、卵巢摘除模型组(OVX组)、阳性对照组(ESV组)、TJC高剂量组(h-TJC组)、TJC中剂量组(m-TJC组)、TJC低剂量组(l-TJC组)。分别于药物干预后的第2周末、第4周末、第6周末、第8周末检测体质量变化;药物干预后第8周末微型计算机断层扫描(micro CT)评估股骨远端干垢端骨质量变化;ELISA法和免疫组化法观测骨代谢标志物变化;qPCR法和Western-blot法检测股骨Wnt5a/β-catenin信号轴关键分子表达水平。结果与SO组比较,OVX组及TJC各剂量组体质量在药物干预后第2周末呈现上升趋势,OVX组、m-TJC组及l-TJC体质量在药物干预后第4周末、第6周末、第8周末也呈现上升趋势,尤以OVX组最为显著;与OVX组比较,h-TJC组体质量在药物干预后第8周末呈现下降趋势。与SO组比较,OVX组股骨远端干垢端骨小梁数目呈现减少趋势,骨小梁间隙呈现增大趋势,并出现空洞现象,BMD和Tb.N呈现减少趋势,Tb.Sp呈现增加趋势;与OVX组比较,h-TJC组动物股骨远端干垢端骨小梁数目接近假手术组,h-TJC组Tb.N呈现增加趋势,Tb.Sp呈现减少趋势,TJC各剂量组BMD呈现增加趋势。与SO组比较,OVX组骨代谢标志物BGP水平呈现下降趋势,TRACP水平呈现上升趋势。与OVX组比较,m-TJC组、h-TJC组BGP水平呈现上升趋势,TJC各剂量组TRACP水平呈现下降趋势。与SO组比较,OVX组Wnt5a/β-catenin信号轴关键分子Wnt5a、β-catenin、Col2α、Runx2表达呈现下调表达趋势,TRACP表达呈现上调表达趋势。与OVX组比较,m-TJC组、h-TJC组Wnt5a/β-catenin信号轴关键分子Wnt5a、β-catenin、Col2α、Runx2 mRNA表达和蛋白表达呈现上调趋势,TRACP mRNA表达呈现下调趋势,TJC各剂量组TRACP蛋白表达也呈现下调趋势。结论TJC通过激Objective To explore the effect of Tenghuang Jiangu Capsule(TJC)on activation of Wnt5a/β-catenin signaling axis on bone metabolism in ovariectomized(OVX)rats.Methods Fifty rats were randomly chosen from sixty SPF-grade rats for the modeling of ovariectomy.The sixty SPF-grade rats were randomly divided into six groups:sham operation group(SO),ovariectomized model group(OVX),positive control group(ESV),TJC high-dose group(h-TJC),TJC middle-dose group(m-TJC),and TJC low-dose group(l-TJC).Body weight was checked on weekends 2,4,6,and 8 after drug intervention.The bone quality changes of the distal femoral metaphysis was evaluated using micro CT on weekend 8 after drug intervention.Enzyme-linked immunosorbent assay(ELISA)and immunohistochemistry(IHC)were used to analyze the expression changes of bone metabolism markers.The expression levels of key molecules in the Wnt5a/β-catenin signaling axis were measured using qPCR and Western blotting.Results Compared to the SO group,the OVX group and all TJC dose groups elevated the body weight on weekend 2 after drug intervention.The body weight was also elevated in the OVX group,m-TJC group,and l-TJC group on weekends 4,6,and 8 after drug intervention,especially in OVX group.The body weight was reduced in h-TJC group on weekend 8 after drug intervention.Compared to those in the SO group,the number of bone trabeculae at the distal femoral metaphysis showed a decreasing trend,the gap between bone trabeculae showed an increasing trend and a phenomenon of cavities,BMD and Tb.N showed a decreasing trend,and Tb.Sp showed an increasing trend.Compared to those in the OVX group,the number of trabeculae at the distal femoral metaphysis in the h-TJC group was similart in the SO group,Tb.N increased,Tb.Sp decreased.BMD showed an upward trend in all doses of TJC groups.Compared to those in the SO group,the levels of BGP showed an downward trend in the OVX group and increased in m-TJC and h-TJC groups.The levels of TRACP showed an upward trend and reduced by all doses of TJC.Compared to t

关 键 词：绝经后骨质疏松 藤黄健骨胶囊 骨代谢 

分 类 号：R681[医药卫生—骨科学]