基于转录组研究哈茨木霉WF2诱导的烟草抗病分子机制  

Transcriptome-based study on the molecular mechanism of Trichoderma harzianum WF2-induced disease resistance in tobacco

作　　者：危潇 黄大野 姚经武 袁勤峰 汪汉成 黎妍妍 曹春霞 WEI Xiao;HUANG Daye;YAO Jingwu;YUAN Qinfeng;WANG Hancheng;LI Yanyan;CAO Chunxia(Hubei Biopesticide Engineering Research Centre,National Biopesticide Engineering Research Center,Wuhan 430064,China;Hubei University of Technology,Wuhan 430068,China;Hubei Academy of Tobacco Science,Wuhan 430030,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China)

机构地区：[1]湖北省生物农药工程研究中心,国家生物农药工程技术研究中心,武汉430064 [2]湖北工业大学,武汉430068 [3]湖北省烟草科学研究院,武汉430030 [4]贵州省烟草科学研究院,贵阳550081

出　　处：《植物保护》2025年第2期44-51,共8页Plant Protection

基　　金：湖北省烟草公司科技项目(027Y2022-005);中国烟草总公司重大科技项目[110202201019(LS-03),110202101048(LS-08),110202001035(LS-04)];湖北省农业科技创新中心创新团队项目(2021-620-000-001-027)。

摘　　要：为了深入探究哈茨木霉WF2诱导烟草抗病性的分子机制,利用转录组测序技术与荧光定量PCR技术,将经过哈茨木霉WF2处理的烟苗与对照的转录组进行对比与分析。结果发现,WF2处理共诱导了377个差异表达基因(DEGs),其中341个DEGs上调表达,36个DEGs下调表达。通过对差异表达基因进行GO和KEGG富集分析,发现WF2主要诱导乙烯(ET)信号通路和MAPK信号通路相关基因的上调表达,其中包括大量植物防御抗性相关基因,如编码ERF、WRKY、TGA等转录因子的基因。此外,哈茨木霉WF2还诱导了非生物胁迫相关的信号调节因子如DREB、CRK、PYL等的上调表达。To further explore the molecular mechanism of Trichoderma harzianum WF2-induced tobacco disease resistance,transcriptome sequencing technology and fluorescence quantitative PCR technology were used to compare and analyze transcriptional level changes between T.harzianum-treated and control groups.The results showed that WF2 treatment induced a total of 377 differentially expressed genes(DEGs),including 341 up-regulated DEGs and 36 down-regulated DEGs.GO and KEGG enrichment analyses revealed that T.harzianum WF2 mainly induced the up-regulated expression of genes related to ethylene(ET)signaling pathway and MAPK signaling pathway,which included numerous plant defense-related genes encoding transcription factors such as ERF,WRKY,and TGA.In addition,T.harzianum WF2 also induced the up-regulation of abiotic stress-related signal regulators like DREB,CRK and PYL.

关 键 词：哈茨木霉 转录组分析 烟草 

分 类 号：S476[农业科学—农业昆虫与害虫防治]