罹病香蕉假茎象甲苏格兰白僵菌的分离鉴定及其生物学特性  

Isolation,identification and biological characteristics of Beauveria caledonica derived from diseased Odoiporus longicollis

作　　者：丁明碧 王永芬 吴丽[1,2] 余宏伟 番华彩 曾莉[2] 徐胜涛[2] 郑泗军[2,4] DING Mingbi;WANG Yongfen;WU Li;YU Hongwei;FAN Huacai;ZENG Li;XU Shengtao;ZHENG Sijun(Resource Plant Research Institute,Yunnan University,Kunming 650500,China;Yunnan Key Laboratory for Green Control of Agricultural Transboundary Pests,Institute of Agricultural Environment and Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Institute of Tropical and Subtropical Economic Crops,Yunnan Academy of Agricultural Sciences,Baoshan 678000,China;Bioversity International,Kunming 650205,China)

机构地区：[1]云南大学资源植物研究院,昆明650500 [2]云南省农业跨境有害生物绿色防控重点实验室,云南省农业科学院农业环境资源研究所,昆明650205 [3]云南省农业科学院热带亚热带经济作物研究所,保山678000 [4]国际生物多样性中心,昆明650205

出　　处：《植物保护》2025年第2期82-96,共15页Plant Protection

基　　金：国家香蕉产业技术体系(CARS-31);云南省万人计划“云岭学者”项目(YNWR-YLXZ-2018-018);国际原子能机构项目(D23033-26673)。

摘　　要：对云南省西双版纳州和昆明市东川区罹病的香蕉假茎象甲Odoiporus longicollis进行了病原分离。通过形态学鉴定及rDNA-ITS序列比对,确定分离到的4个菌株均为苏格兰白僵菌Beauveria caledonica。通过对这4个分离株的离体培养条件探索及毒力测定,表明离体培养的最佳条件为光周期L∥D=24 h∥0 h、温度25℃、在加有虫粉的PDA培养基上培养18 d,此条件下的4个菌株菌落直径和产孢量最高,其中Cs-1达到(65.17±0.74)mm和1.01×10^(8)个/cm^(2),乳糖和牛肉膏分别为最适碳、氮源。生物测定结果表明,Cs-1菌株在接种浓度为1×10^(9)个/mL时对香蕉假茎象甲致死中时(LT_(50))最短,为9.254 d,致死中浓度(LC_(50))为3.618×10^(6)个/mL,20 d后的致死率为86.67%。本研究首次在云南蕉园发现并鉴定的4株昆虫病原真菌均为苏格兰白僵菌,Cs-1菌株对香蕉假茎象甲具有较好的生防潜力,可望今后应用于香蕉假茎象甲的生物防治。Four fungal strains were isolated from infected Odoiporus longicollis collected in Jinghong city,Xishuangbanna prefecture and Dongchuan district in Kunming city,Yunnan province.They were identified as Beauveria caledonica based on morphological characteristics and rDNA-ITS sequence analysis.The biological characteristics of the strains under different conditions(culture temperature,light duration,medium composition,pH,carbon and nitrogen sources)and their pathogenicity to O.longicollis were investigated.The results showed that 25℃,L∥D=24 h∥0 h,18 days of culture on PDA medium supplemented with insect meal were optimal cultured condition with the maximum colony diameter and spore production of(65.17±0.74)mm and 1.01×10^(8)spores/cm^(2),respectively,for Cs-1.The most suitable carbon and nitrogen sources were lactose and beef paste.Bioassays revealed that the Cs-1 strain,at an inoculation concentration of 1×10^(9)spores/mL,achieved an LT_(50) of 9.254 days,an LC_(50) of 3.618×10^(6)spores/mL,and a cumulative mortality rate of 86.67%after 20 days.This study marks the first identification of B.caledonica strains in banana plantations in Yunnan.The Cs-1 strain demonstrates significant potential as a biocontrol agent against O.longicollis and warrants further investigation for field application.

关 键 词：香蕉假茎象甲 苏格兰白僵菌 生物学特性 致病力 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]