METTL3-Mediated LINC00475 Alternative Splicing Promotes Glioma Progression by Inducing Mitochondrial Fission  

作　　者：Yaping Yan Ailing Luo Shanshan Liu Mansi Cai Xiaodan Liu Xiaohong Zhang Siyi Zhang Yu Liu Jiamin Zeng Xinke Xu Na Zhang Zhuorong Zhang Yingyi Xu Jing He Xiaoping Liu 

机构地区：[1]Department of Hematology and Oncology,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou 510623,China [2]Division of Birth Cohort Study,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou 510623,China [3]Department of Anesthesiology,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou 510623,China [4]Department of Nephrology,Center of Kidney and Urology,The Seventh Affiliated Hospital,Sun Yat-sen University,Shenzhen 518107,China [5]Department of Anesthesiology,The Second Affiliated Hospital of University of South China,Hengyang,Hunan Province 421001,China [6]Department of Neurosurgery,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou 510623,China [7]Department of Pediatric Surgery,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou 510623,China [8]Department of Pediatric Surgery,Guangzhou Institute of Pediatrics,Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou 510623,China

出　　处：《Research》2024年第4期348-364,共17页研究(英文)

基　　金：supported by the National Natural Science Foundation of China(nos.81672496 and no.82173593);Guangzhou Municipal Science and Technology Bureau(nos.202201020603 and 202201020640);Guangzhou Municipal Science and Technology Program key projects(no.202206010006).

摘　　要：Mitochondrial fission promotes glioma progression.The function and regulation mechanisms of lncRNAs in glioma mitochondrial fission are unclear.The expression of LINC00475 and its correlation with clinical parameters in glioma were analyzed using bioinformatics.Then,in vitro and in vivo assays were performed to explore the function of spliced variant LINC00475(LINC00475-S)in gliomas.To explore the mechanisms,RNA-seq,MeRIP,RIP,pulldown-IP,dCas9-ALKBH5 editing system,LC/MS,and Western blotting were utilized.LINC00475 was confirmed to be overexpressed and with higher frequencies of AS events in gliomas compared to normal brain tissue and was associated with worse prognosis.In vitro and animal tumor formation experiments demonstrated that the effect of LINC00475-S on proliferation,metastasis,autophagy,and mitochondrial fission of glioma cells was significantly stronger than that of LINC00475.Mechanistically,METTL3 induced the generation of LINC00475-S by splicing LINC00475 through m6A modification and subsequently promotes mitochondrial fission in glioma cells by inhibiting the expression of MIF.Pull-down combined LC/MS and RIP assays identified that the m6A recognition protein HNRNPH1 bound to LINC00475 within GYR and GY domains and promoted LINC00475 splicing.METTL3 facilitated HNRNPH1 binding to LINC00475 in an m6A-dependent manner,thereby inducing generation of LINC00475-S.METTL3 facilitated HNRNPH1-mediated AS of LINC00475,which promoted glioma progression by inducing mitochondrial fission.Targeting AS of LINC00475 and m6A editing could serve as a therapeutic strategy against gliomas.

关 键 词：promoted thereby utilized 

分 类 号：O57[理学—粒子物理与原子核物理]