lncRNA PCAT19对胃癌细胞MGC-803增殖和凋亡的影响及作用机制  

Effect and mechanism of lncRNA PCAT19 on proliferation and apoptosis of MGC-803 gastric cancer cells

作　　者：骆曼[1] 胡作为[1] 肖柳 LUO Man;HU Zuowei;XIAO Liu(Department of Oncology,Wuhan NO.1 Hospital,Wuhan 430000,China)

机构地区：[1]武汉市第一医院肿瘤科,武汉430000

出　　处：《药学前沿》2025年第3期381-387,共7页China Pharmacist

基　　金：武汉市卫生健康委科研项目(WZ22C40);湖北省中医药管理局中医药科研项目(ZY2021M093)。

摘　　要：目的检测长链非编码RNA(lncRNA)PCAT19在胃癌组织和细胞株中的表达水平,并探讨其对胃癌细胞增殖、凋亡的影响及作用机制。方法采用RT-qPCR检测胃癌组织及3种胃癌细胞株(MKN-45、BGC-823、MGC-803)中lncRNA PCAT19的表达水平。对胃癌组织中lncRNA PCAT19的表达水平与患者临床病理特征进行相关性分析。通过si-PCAT19转染干扰MGC-803细胞中lncRNA PCAT19表达,CCK-8实验检测细胞增殖情况,流式细胞术检测细胞凋亡情况,Western blotting实验检测对增殖和凋亡相关蛋白表达的影响。结果与癌旁组织相比,胃癌组织中lncRNA PCAT19的表达水平明显升高(P<0.05),且其表达水平与胃癌浸润程度呈正相关(P<0.05)。与人胃黏膜上皮细胞GES-1相比,3株胃癌细胞中lncRNA PCAT19的表达水平均明显升高(P<0.05)。与si-NC组比较,si-PCAT19组MGC-803细胞的增殖能力降低(P<0.05),凋亡能力升高(P<0.05),细胞周期蛋白D1(Cyclin D1)蛋白表达降低(P<0.05),天冬氨酸特异性的半胱氨酸蛋白水解酶9(Caspase-9)蛋白表达升高(P<0.05)。结论lncRNA PCAT19在胃癌组织和细胞中高表达,下调lncRNA PCAT19能抑制胃癌细胞增殖并促进凋亡,其机制可能和调控Cyclin D1、Caspase-9蛋白表达有关。Objective To investigate the expression level of lncRNA PCAT19 in gastric cancer tissues and cell lines,and determine its effect and mechanism on the proliferation and apoptosis of gastric cancer cells.Methods RT-qPCR was used to detect the expression level of lncRNA PCAT19 in gastric cancer tissues and three gastric cancer cell lines(MKN-45,BGC-823 and MGC-803).The correlation between the expression level of lncRNA PCAT19 in gastric cancer tissues and the clinical pathological characteristics of patients was analyzed.lncRNA PCAT19 expression in MGC-803 cells was interfered by si-PCAT19 transfection,and the changes in the proliferation and apoptosis of cells were investigated using a CCK-8 kit and flow cytometry,respectively.The effect on expression level of proliferation and apoptosis-related proteins was detected by Wstern blot assay.Results Compared with adjacent tissues,the expression level of lncRNA PCAT19 in gastric cancer tissues was significantly higher(P<0.05),and its expression level was positively correlated with the degree of gastric cancer invasion(P<0.05).Compared with human gastric mucosal epithelial cells GES-1,the expression levels of lncRNA PCAT19 in three gastric cancer cell lines were significantly higher(P<0.05).Compared with the si-NC group,the proliferation ability of MGC-803 cells in the si-PCAT19 group was reduced(P<0.05),the apoptotic ability was increased(P<0.05),and the expression level of Cyclin D1 was reduced(P<0.05),while the expression level of Caspase-9 was increased(P<0.05).Conclusion lncRNA PCAT19 is highly expressed in gastric cancer tissues and cells.Downregulation of lncRNA PCAT19 can inhibit cell proliferation and promote cell apoptosis,which may be related to its regulation of Cyclin D1 and Caspase-9 protein expression.

关 键 词：胃癌 长链非编码RNA PCAT19 增殖 凋亡 机制 

分 类 号：R34[医药卫生—基础医学]