利用基因组规模数据推断臭虫次目的高阶元系统发育关系  

Inference of higher-level phylogenetic relationships in Cimicomorpha using the genome-scale data

作　　者：林兴雨 贾征楠 田洪云 李萌 宋南[1] LIN Xing-Yu;JIA Zheng-Nan;TIAN Hong-Yun;LI Meng;SONG Nan(Henan International Laboratory for Green Pest Control,Henan Engineering Laboratory of Pest Biological Control,College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,China;Yunnan Fortmeta Biotech Co.,Ltd.,Yuxi 652500,China;Yuqing County Tobacco Monopoly Bureau(Branch),Yuqing 564400,China;College of Tobacco Science and Engineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China)

机构地区：[1]河南农业大学植物保护学院,河南省害虫绿色防控国际联合实验室,河南省害虫生物防控工程实验室,郑州450046 [2]云南峰芒生物科技有限责任公司,玉溪652500 [3]余庆县烟草专卖局(分公司),余庆564400 [4]郑州轻工业大学烟草科学与工程学院,郑州450001

出　　处：《昆虫学报》2025年第2期213-222,共10页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(U1904104)。

摘　　要：【目的】利用基因组和转录组数据构建臭虫次目(Cimicomorpha)高阶元(科或总科)的系统发育关系,以期为进一步明确臭虫次目的高阶元系统发育关系提供数据。【方法】通过高通量测序技术获取了梨冠网蝽Stephanotis nashi的基因组数据,并将其与已经发表的异翅亚目(Heteroptera)其他38个种的基因组和转录组数据相结合。利用软件BUSCO提取上述物种的单拷贝直系同源基因,并构建了6个不同完整性的数据矩阵,以研究臭虫次目的高阶元系统发育关系。【结果】臭虫次目单拷贝直系同源基因的数量介于397~2437个之间。在臭虫次目的系统发育关系中,所有结果都支持猎蝽总科(Reduvioidea)、姬蝽总科(Naboidea)、臭虫总科(Cimicoidea)、网蝽总科(Tingoidea)和盲蝽总科(Miroidea)作为单系群;猎蝽总科独立成一支,并与由姬蝽总科、臭虫总科、网蝽总科及盲蝽总科所组成的支系形成姐妹群关系。此外,科级阶元的系统分析结果揭示猎蝽科(Reduviidae)、姬蝽科(Nabidae)、花蝽科(Anthocoridae)、网蝽科(Tingidae)和盲蝽科(Miridae)均为单系群,并支持网蝽科和盲蝽科为姐妹群关系。【结论】本研究结果说明,利用基因组和转录组数据在构建臭虫次目的系统发育关系的实用性,也为深入理解臭虫次目高阶元的系统发育关系提供了基因组和转录组数据。【Aim】The phylogenetic relationships among higher taxonomic groups(family or superfamily)within Cimicomorpha were reconstructed using genome and transcriptome data,with the aim of providing data to further clarify higher-level phylogenetic relationships within Cimicomorpha.【Methods】Through high-throughput sequencing technology,we successfully obtained the genome data of Stephanotis nashi and integrated them with publicly available genome and transcriptome data from 38 other species of Heteroptera.Using the software BUSCO,we extracted single-copy orthologous genes from the above species,and constructed six data matrices of varying completeness to study the higher-level phylogenetic relationships within Cimicomorpha.【Results】The number of single-copy orthologous genes in Cimicomorpha ranged from 397 to 2437.In Cimicomorpha,phylogenetic analyses consistently supported the monophyly of Reduvioidea,Naboidea,Cimicoidea,Tingoidea,and Miroidea.The Reduvioidea group formed an independent clade and was sister to a clade consisting of Naboidea,Cimicoidea,Tingoidea,and Miroidea.Additionally,at the family level,the phylogenetic analysis indicated that the families Reduviidae,Nabidae,Anthocoridae,Tingidae and Miridae each represented a monophyletic group,and the families Tingidae and Miridae were sister groups.【Conclusion】The results of this study demonstrate the practicality of using genome and transcriptome data in constructing the phylogenetic relationships within Cimicomorpha,and also provide genome and transcriptome data to further understand the higher-level phylogenetic relationships in Cimicomorpha.

关 键 词：半翅目 臭虫次目 基因组 转录组 单拷贝直系同源基因 系统发育 

分 类 号：Q969.35[生物学—昆虫学]