分泌性中耳炎穿刺液中p38 MAPK/NF-κB/NLRP3信号通路蛋白表达及与术后复发的相关性  

Expression of p38 mitogen-activated protein kinase(p38 MAPK),nuclear transcription factor-κB(NF-κB),and NOD-like receptor thermal protein domain associated protein 3(NLRP3)signalling pathway proteins in perforated fluid of secretory otitis media and correlation with postoperative recurrence

作　　者：吴沭城 庄汉 岳苏苏 WU Shucheng;ZHUANG Han;YUE Susu(Department of Otorhinolaryngology,Shuyang Affiliated Hospital,Nanjing University of Traditional Chinese Medicine,Shuyang Jiangsu 223600,China;Department of Otorhinolaryngology,Suqian Hospital,Nanjing Gulou Hospital Group,Nanjing 223800,China)

机构地区：[1]南京中医药大学沭阳附属医院耳鼻喉科,江苏沭阳223600 [2]南京鼓楼医院集团宿迁医院耳鼻喉科,南京223800

出　　处：《新疆医科大学学报》2025年第3期293-298,共6页Journal of Xinjiang Medical University

基　　金：江苏省科技项目(BK20211235)。

摘　　要：目的分析p38丝裂原活化蛋白激酶(p38 MAPK)/核转录因子-κB(NF-κB)/NOD样受体蛋白3(NLRP3)信号通路蛋白在分泌性中耳炎(SOM)黏膜组织中的表达情况及与患者术后复发的相关性。方法回顾性分析2022年7月—2024年1月南京中医药大学沭阳附属医院耳鼻喉科收治的42例行鼓膜穿刺抽液术治疗的SOM患者的临床资料。术后随访6个月,以患者是否复发为分组依据,将患者分为复发组和未复发组。比较两组患者基本资料、p38 MAPK/NF-κB/NLRP3信号通路蛋白表达情况。分析SOM患者术后复发的影响因素,绘制受试者工作特征(ROC)曲线,分析p38 MAPK/NF-κB/NLRP3信号通路蛋白单一及联合预测SOM患者术后的效能。结果复发组患者通气管保留时间(<3个月)的占比和p38 MAPK、NF-κB、NLRP3信号通路蛋白表达量均高于未复发组,差异有统计学意义(P<0.05)。以SOM患者术后是否复发作为因变量,以通气管保留时间、p38 MAPK、NF-κB、NLRP3作为自变量,二元Logistic回归分析结果显示,通气管保留时间<3个月、p38 MAPK、NF-κB、NLRP3均是SOM患者术后复发的独立影响因素(P<0.05)。p38 MAPK、NF-κB、NLRP3联合预测SOM患者术后复发的灵敏度、特异度、AUC均高于各指标单一预测(P均<0.05)。结论通气管保留时间较短、p38 MAPK/NF-κB/NLRP3信号通路蛋白表达异常可能是引起穿刺抽液治疗SOM术后复发的独立因素,联合p38 MAPK/NF-κB/NLRP3信号通路蛋白预测SOM效能较高,临床可作为参考依据。Objective To analyse the expression of p38 mitogen-activated protein kinase(p38 MAPK)/nuclear transcription factor-κB(NF-κB)/NOD-like receptor protein 3(NLRP3)signaling pathway proteins in mucosal tissues of secretory otitis media(SOM)and the correlation with the patients'postoperative recurrence.Methods The clinical data of 42 cases of SOM patients treated with tympanocentesis and fluid extraction admitted to the hospital from July 2022 to January 2024 were reviewed.The patients were followed up for 6 months after the operation,and were divided into 13 cases in the recurrence group and 29 cases in the non-recurrence group on the basis of the patients'recurrence status as a grouping basis.The basic data,p38 MAPK/NF-κB/NLRP3 signalling pathway protein expression were compared between the 2 groups.The influencing factors of post operative recurrence in SOM patients were analysed,and ROC curves were plotted to analyse the efficacy of p38 MAPK,NF-κBand NLRP3 signalling pathway proteins singly and jointly in predicting the efficacy of post operative SOM patients.Results The percentage of thepatients with ventilation tube retention time(<3 months),the protein expression of p38 MAPK,NF-κB and NLRP3 signalling pathway in the recurrence group was significantly higher than that in the non-recurrence group,and the difference was statistically significant(P<0.05).Binary-Logistic-regression analysis was performed with whether SOM patients had postoperative recurrence as the dependent variable,and ventilation tube retention time,p38 MAPK,NF-κB and NLRP3 as the independent variables.The results showed that ventilation tube retention time<3 months,p38 MAPK,NF-κB and NLRP3 were all independent influences on the postoperative recurrence in SOM patients The sensitivity,specificity and AUC of p38 MAPK,NF-κB and NLRP3 in combining to predict postoperative recurrence in SOM patients were higher than those of each index alone(all P<0.05).Conclusion Shorter tracheal retention time and abnormal expression of p38 MAPK,NF-κB and N

关 键 词：分泌性中耳炎 信号通路 复发 相关性 

分 类 号：R764[医药卫生—耳鼻咽喉科]