保存温度对不同季节秦岭石生苔藓种源生理活性的影响  

Effects of Preservation Temperature on Physiological Activity of Saxicolous Bryophyte Crust Provenances in Different Seasons of Qinling Mountains

作　　者：王子临 张学锋 张志全 杨梦茹 拓行行 王亦波 田慧慧 尹子鸣 马晓瑞 卜崇峰[3] 李伟[3] WANG Zi-lin;ZHANG Xue-feng;ZHANG Zhi-quan;YANG Meng-ru;TUO Hang-hang;WANG Yi-bo;TIAN Hui-hui;Yin Zi-ming;MA Xiao-rui;BU Chong-feng;LI Wei(College of Grassland Agriculture,Northwest A&F University,Yangling,Shaanxi Province 712100,China;Sichuan Yanjiang Yijin Expressway Co.,Ltd.,Xichang,Sichuan Province 615000,China;College of Soil and Water Conservation Science and Engineering,Northwest A&F University,Yangling,Shaanxi Province 712100,China)

机构地区：[1]西北农林科技大学草业与草原学院,陕西杨凌712100 [2]四川沿江宜金高速公路有限公司,四川西昌615000 [3]西北农林科技大学水土保持科学与工程学院,陕西杨凌712100

出　　处：《草地学报》2025年第3期678-685,共8页Acta Agrestia Sinica

基　　金：国家重点研发计划项目(2022YFF1302800);国家自然科学基金项目(42277464);陕西省重点研发项目(2024SF-YBXM-545);四川省交通运输科技项目(2018-ZL-15)资助。

摘　　要：苔藓结皮作为生物土壤结皮的顶级演替阶段,具有涵养水源、修复地表生态、防治水土流失和促进灌草定植的功能。羽枝青藓(Brachythecium plumosum(Hedw.)B.S.G.)作为秦岭北麓裸石表面广泛分布的优势石生藓种,其繁殖利用效率对湿度、温度等微环境变化极其敏感。为明确秦岭石生苔藓结皮干燥保存的最佳温度,本研究以羽枝青藓为主要建群种的秦岭石生苔藓结皮作为研究对象,分别测定了四季采集的羽枝青藓在不同温度(-5℃、5℃、15℃、25℃)遮光干燥保存下的可溶性糖、可溶性蛋白、丙二醛和叶绿素a、b含量等指标,并通过曲线拟合预测了羽枝青藓生理指标的变化趋势。结果表明:羽枝青藓石生苔藓结皮的最佳采集季节为秋季,最佳保存温度处理组为5℃,模拟预测最佳保存温度为2.42℃。该研究结果为苔藓结皮保存、工厂化生产及生态修复应用提供了重要理论和实践依据。Moss crusts,as the ultimate stage of biological soil crust succession,are capable of conserving water sources,repairing surface ecosystems,preventing soil erosion,and promoting the establishment of shrubs and grasses.Brachythecium plumosum(Hedw.)B.S.G.,a dominant bryophyte species widely distributed on the bare rocks of the northern slopes of the Qinling Mountains,is extremely sensitive to microenvironmental change such as humidity and temperature in terms of its reproductive utilization efficiency.To clarify the opti⁃mal temperature for the dry preservation of saxicolous bryophyte crust in the Qinling Mountains,in this study we took the saxicolous bryophyte crust dominated by Brachythecium plumosum as the research subject,and measured contents of soluble sugar,soluble protein,malondialdehyde,and chlorophyll a and b of Brachythe⁃cium plumosum thant collected in different seasons under dry preservation in shade and at various temperatures(-5°C,5°C,15°C,25°C).Curve fitting was also used to predict the trends in physiological indicators of Brachythecium plumosum.The results showed that the optimal collection season for saxicolous bryophyte crust dominated by Brachythecium plumosum is autumn,and the best preservation temperature treatment is 5°C,with the simulated prediction for the best preservation temperature is 2.42°C.The results of this study provide an important theoretical and practical basis for the industrial preservation,production and ecological restoration application of moss crust.

关 键 词：生物土壤结皮 苔藓结皮 秦岭石生苔藓 羽枝青藓 苔藓结皮保存 

分 类 号：Q145.2[生物学—生态学]