海雀稗HsFA基因家族鉴定及热胁迫应答分析  

Identification of HsFA Family Genes and Expression Profiling in Paspalum vaginatum under Heat Stress Conditions

作　　者：彭迪 陈海洋 蔡丽蓉 陆洋 刘大林[1] 王小山[1] 严学兵 潘玲[1] PENG Di;CHEN Hai-yang;CAI Li-rong;LU Yang;LIU Da-lin;WANG Xiao-shan;YAN Xue-bing;PAN Ling(Yangzhou University,College of Animal Science and Technology,Yangzhou,Jiangsu Province 225000,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225000

出　　处：《草地学报》2025年第3期766-777,共12页Acta Agrestia Sinica

基　　金：海南省国家自然科学基金青年基金一项(322QN248);扬州大学海内外优秀人才科研启动经费(137013098/5019);国家自然科学基金(32401488)资助。

摘　　要：本研究采用生物信息学方法鉴定海雀稗(Paspalum vaginatum Sw.)中的热激转录因子PvHsFA家族基因成员,并探究其在海雀稗热胁迫应答中的调控机制,旨在提供相关理论依据。通过生物信息学手段鉴定了PvHsFA基因家族的成员,并分析这些成员的理化性质、启动子区域的顺式作用元件、构建进化树以及进行染色体定位。此外,利用海雀稗品种‘海岛2000’的RNA-seq数据对基因表达水平进行了验证。共鉴定出25个PvHsFA家族成员,其编码氨基酸数目261~521,这些蛋白大多为酸性蛋白和亲水性蛋白。启动子区域含有大量与非生物胁迫相关、植物激素相关及生长发育相关的元件。这些基因具有较高的保守性,其中21个基因在热胁迫条件下表现出显著的差异表达。PvHsFA基因家族可能在海雀稗的热胁迫响应调控中发挥重要作用,本研究为深入揭示海雀稗的耐热机制奠定了基础。This study aims to perform a bioinformatics analysis to identify and characterize the PvHsFA gene family of heat stress transcription factors in seashore paspalum(Paspalum vaginatum Sw.).The goal is to offer theoretical insights into the regulatory mechanisms of this gene family during the heat stress response in seashore paspalum.We employed bioinformatics tools to discover and to classify members of the PvHsFA gene family.We then analyzed their physicochemical properties,the presence of cis-acting elements within their pro⁃moter sequences,constructed evolutionary trees,and mapped their chromosomal locations.To validate our findings,RNA-sequencing data from‘Sealsle 2000’cultivar of seashore paspalum were utilized.Our analysis identified a total of 25 PvHsFA gene family members.The proteins varied in size,with the number of coding amino acids ranging from 261 to 521,and the majority exhibited characteristics of being acidic and hydrophilic.The promoters of these genes were rich in hormone-responsive,stress-responsive,and growth-related ele⁃ments.Expression profiling indicated that 21 of the genes were actively expressed.The findings suggest that the PvHsFA gene family may play a pivotal role in mediating the heat stress response in seashore paspalum.This research establishes a foundation for future investigations into the heat tolerance mechanisms of this eco⁃logically significant grass species.

关 键 词：生物信息学 HsFA基因家族 海雀稗 转录因子 热胁迫 基因表达 

分 类 号：S688.4[农业科学—观赏园艺]