基于LC-MS/MS技术测定类风湿性关节炎患者血浆中微量氨甲蝶呤浓度方法学的建立  

Development of an assay for measurement of serum trace methotrexate in patients with rheumatoid arthritis by LC-MS/MS

作　　者：郑彩云 林妹妹[1] 陈旭[1] ZHENG Cai-yun;LIN Mei-mei;CHEN Xu(Fuqing Hospital Affiliated to Fujian Medical University,Fuqing,Fujian 350300)

机构地区：[1]福建医科大学附属福清市医院,福建福清350300

出　　处：《赣南医科大学学报》2025年第2期124-129,共6页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：福州市卫生健康科技计划项目(2022-S-wq1);福建医科大学启航基金项目(2020QH1346)。

摘　　要：目的:建立液相色谱-串联质谱(Liquid chromatography-tandem mass spectrometry,LC-MS/MS)法测定类风湿性关节炎(Rheumatoid arthritis,RA)患者血浆中微量氨甲蝶呤的浓度。方法:以氨甲蝶呤-d3为内标、色谱柱为Agilent EC-C_(18)(2.1 mm×50 mm,2.7μm),流动相为0.1%甲酸-水和乙腈,梯度洗脱,流速:0.3 mL·min^(-1);柱温:40℃。结果:氨甲蝶呤保留时间为2.939 min;氨甲蝶呤血浆样品线性范围为0.0012~3μmol·L^(-1)(r^(2)=0.9999)。结论:本研究建立的LC-MS/MS技术测定RA患者血浆中微量氨甲蝶呤浓度的方法准确、简单、快速,可为临床个体化用药提供依据。Objective:To develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)method capable for measuring serum methotrexate in RA patients.Methods:With methotrexate-d3,the chromatography was performed on Agilent EC-C18(2.1 mm×50 mm,2.7μm)column with mobile phase consisting of 0.1%formic acid-water and acetonitrile.The flow rate was 0.3 mL·min-1.Column temperature:40℃.Results:The retention time of methotrexate was 2.939 min.The linear range of methotrexate plasma samples was 0.0012~3μmol·L-1(r2=0.9999).Conclusion:The LC-MS/MS method established in this study is accurate,simple and rapid.It could provide basis for clinical individualized medication.

关 键 词：低剂量氨甲蝶呤 血药浓度 液相色谱-串联质谱 方法学 

分 类 号：R593.22[医药卫生—内科学]