3D bioprinted chondrogenic gelatin methacrylate-poly(ethylene glycol)diacrylate composite scaffolds for intervertebral disc restoration  

作　　者：Maria D Astudillo Potes Maryam Tilton Indranath Mitra Xifeng Liu Babak Dashtdar Emily T Camilleri Benjamin D Elder Lichun Lu 

机构地区：[1]Mayo Clinic Alix School of Medicine,Rochester,MN,United States of America [2]Mayo Clinic Graduate School of Biomedical Sciences,Rochester,MN,United States of America [3]Department of Physiology and Biomedical Engineering,Mayo Clinic,Rochester,MN,United States of America [4]Department of Orthopedic Surgery,Mayo Clinic,Rochester,MN,United States of America [5]Department of Neurological Surgery,Mayo Clinic,Rochester,MN,United States of America [6]Walker Department of Mechanical Engineering,The University of Texas at Austin,Austin,TX,United States of America

出　　处：《International Journal of Extreme Manufacturing》2025年第1期610-621,共12页极端制造(英文)

基　　金：supported by NIH Grant(T32GM065841),Mayo Foundation for Education and Research.

摘　　要：Degenerative spine pathologies,including intervertebral disc(IVD)degeneration,present a significant healthcare challenge due to their association with chronic pain and disability.This study explores an innovative approach to IVD regeneration utilizing 3D bioprinting technology,specifically visible light-based digital light processing,to fabricate tissue scaffolds that closely mimic the native architecture of the IVD.Utilizing a hybrid bioink composed of gelatin methacrylate(GelMA)and poly(ethylene glycol)diacrylate(PEGDA)at a 10%concentration,we achieved enhanced printing fidelity and mechanical properties suitable for load-bearing applications such as the IVD.Preconditioning rat bone marrow-derived mesenchymal stem cell spheroids with chondrogenic media before incorporating them into the GelMA-PEGDA scaffold further promoted the regenerative capabilities of this system.Our findings demonstrate that this bioprinted scaffold not only supports cell viability and integration but also contributes to the restoration of disc height in a rat caudal disc model without inducing adverse inflammatory responses.The study underscores the potential of combining advanced bioprinting techniques and cell preconditioning strategies to develop effective treatments for IVD degeneration and other musculoskeletal disorders,highlighting the need for further research into the dynamic interplay between cellular migration and the hydrogel matrix.

关 键 词：intervertebral disc regeneration 3D bioprinting gelatin-based hydrogels mesenchymal stem cell spheroids tissue engineering 

分 类 号：R31[医药卫生—基础医学]