山羊OR2AP1基因多态性及其与体尺性状的关联分析  

Polymorphism of OR2AP1 gene in goats and its association with body size traits

作　　者：符明永 赵秀慧 刘杰[1] 董书餐 柳广斌[1] 邓铭[1] 郭勇庆 孙宝丽[1] 刘德武[1] 李耀坤[1] FU Mingyong;ZHAO Xiuhui;LIU Jie;DONG Shucan;LIU Guangbin;DENG Ming;GUO Yongqing;SUN Baoli;LIU Dewu;LI Yaokun(College of Animal Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《畜牧与兽医》2025年第4期26-35,共10页Animal Husbandry & Veterinary Medicine

基　　金：广东省科技特派员项目(KTP20210344);广东省现代农业产业技术体系项目(2023KJ127)。

摘　　要：为得到一个或多个分子选育种标记,以加快生长性能好、产肉效率高的山羊选育工作进程。以雷州山羊、川中黑山羊、努比亚山羊为试验对象,选取健康成年母羊进行体尺测量和血液样品采集,通过扩增OR2AP1基因、DNA测序和PCR-RFLP检测OR2AP1基因的多态性,利用SnapGene软件查找其所对应的限制性内切酶,使用IBM SPSS Statistics 26统计分析软件程序配合构建的单位点效应模型对山羊各位点进行统计分析,研究OR2AP1基因多态性与体尺性状的关联性。结果:OR2AP1基因上有8个单核苷酸多态性(SNP):g.94G>A、g.115G>A、g.322C>T、g.349C>T、g.357C>T、g.365T>C、g.627G>A和g.909C>T;影响体高、体直长和体斜长的位点分别为g.115G>A、g.627G>A、g.627G>A;影响胸围的位点为g.115G>A和g.357C>T,影响后腿围的位点为g.115G>A和g.357C>T,影响管围的位点为g.357C>T和g.627G>A;影响尻宽的位点为g.115G>A、g.357C>T、g.365C>T;通过连锁不平衡分析发现,影响山羊体尺性状最佳组合单倍型为g.357C>T和g.365T>C位点上CCCT、TTTT型,g.115G>A和g.357C>T位点上AATT、GATT型。体高最佳组合单倍型为CCCT、AATT,体直长、胸围、尻宽最佳组合单倍型分别为GATT、AATT、TTTT。综上,g.115G>A、g.357C>T、g.365C>T、g.627G>A可作为改良山羊体尺性状的分子标记;CCCT、TTTT型(g.357C>T和g.365T>C)以及AATT、GATT型(g.115G>A和g.357C>T)是培育优秀体尺性状山羊的优势单倍型。The aim of this study was to obtain one or more molecular selection markers and accelerate the process of goat selection and breeding with good growth performance and high meat production efficiency.Healthy adult ewes were selected for body size measurement and blood sample collection,the polymorphisms of OR2AP1 gene were detected by amplification of OR2AP1 gene,DNA sequencing and PCRRFLP,the corresponding restriction enzymes were found by SnapGene software,and the unit point effect model constructed by IBM SPSS Statistics 26 was used to statistically analyze the goat sites to study the association between OR2AP1 gene polymorphisms and body size traits.The results showed that there were eight SNPs in the OR2AP1 gene:g.94G>A,g.115G>A,g.322C>T,g.349C>T,g.357C>T,g.365T>C,g.627G>A and g.909C>T.The loci affecting body height,body length and body oblique length were g.115G>A,g.627G>A,and g.627G>A,respectively;and the loci affecting chest circumference,hind leg circumference,and tube circumference were g.115G>A,g.>357C>T,g.357CT,and g.627G>A,respectively;and g.115G>A,g.357C>T,and g.627G>A,respectively;and g.115G>A,g.357C>T,g.365C>T,respectively.The linkage disequilibrium analysis showed that the haplotype of the best combination of body size traits affecting goat were g.357C>T and g.365T>C:CCCT,TTTT;and g.115G>A and g.357C>T:AATT,GATT.The best combination haplotype of body height were CCCT and AATT,and the best combination haplotype of body length,chest circumference and jiri width were GATT,AATT and TTTT,respectively.In conclusion,g.115G>A,g.357C>T,and g.365C>T and g.627G>A could be used as molecular markers for improved goat body size traits,while CCCT,TTTT(g.357C>T and g.365T>C),AATT and GATT(g.115G>A and g.357C>T)were the dominant haplotype for breeding goats with excellent body size traits.

关 键 词：SNP 遗传多样性 雷州山羊 川中黑山羊 努比亚山羊 

分 类 号：S826[农业科学—畜牧学]