苏州地区育龄期健康单胎妊娠女性妊娠期血清泌乳素参考区间的建立与验证  

Establishment and Verification of Reference Interval of Serum Prolactin in Healthy Single Pregnant Women of Childbearing Age in Suzhou,China

作　　者：孙芳璨 李丽[2] 李晓宇 周金华[1] 韩冰[1] SUN Fangcan;LI Li;LI Xiaoyu;ZHOU Jinhua;HAN Bing(Department of Obstetrics and Gynecology,The First Affiliated Hospital of Soochow University,Suzhou,Jiangsu 215006,China;Department of Obstetrics and Gynecology Laboratory,The First Affiliated Hospital of Soochow University,Suzhou,Jiangsu 215006,China)

机构地区：[1]苏州大学附属第一医院妇产科,苏州215006 [2]苏州大学附属第一医院妇产科实验室,苏州215006

出　　处：《协和医学杂志》2025年第2期393-398,共6页Medical Journal of Peking Union Medical College Hospital

基　　金：苏州大学附属第一医院自然科学基金博习培育计划(BXQN202222)。

摘　　要：目的分析苏州地区育龄期健康单胎妊娠女性妊娠期血清泌乳素(prolactin,PRL)水平,建立并验证其参考区间。方法收集2022年1—3月在苏州大学附属第一医院产科门诊建卡行产前检查的育龄期健康单胎妊娠女性资料,前瞻性随访至分娩。根据孕周将研究对象分为早孕期组(妊娠<14周)、中孕期组(14~27^(+6)周)和晚孕期组(≥28周)。采用索灵LIAISON XL全自动化学发光免疫分析仪及LIAISON促乳素测定试剂盒测定其血清PRL水平,剔除离群值后采用百分位数法(P_(2.5)~P_(97.5))建立妊娠期血清PRL医学参考区间。随机选取早、中、晚孕期育龄期健康单胎妊娠女性各20例验证参考范围,当每组不超过2例PRL测定结果超出所建立的参考区间视为通过验证。结果早孕期组170人、中孕期组229人、晚孕期组130人纳入本研究。在不同妊娠阶段,孕妇血清PRL水平存在显著差异,且随着孕周的增加,其水平呈上升状态。早孕期、中孕期和晚孕期的血清PRL参考区间分别为477~4270 mIU/L、1060~6574 mIU/L、3497~18274 mIU/L,建立的参考区间均通过了验证。结论本研究建立了苏州地区育龄期健康单胎妊娠女性妊娠期血清PRL的参考区间,为临床合理应用该指标提供了帮助,有望进一步为预防妊娠相关疾病提供参考依据。Objective To analyze serum prolactin(PRL)levels during pregnancy in healthy single pregnant women of childbearing age in Suzhou,and to establish and verify the reference interval of serum PRL.Methods From January to March,2022,the data of pregnant women with healthy single pregnancy at child⁃bearing age were collected and prospectively followed up until delivery.According to the gestational age,the subjects were divided into early pregnancy group(less than 14 weeks),middle pregnancy group(14-27^(+6) weeks)and late pregnancy group(≥28 weeks).PRL was determined by Soling LIAISON XL automatic chemi⁃luminescence immunoassay and LIAISONprolactin.After eliminating outliers,the medical reference interval of serum PRL during pregnancy was established by percentile method(P_(2.5)-P_(97.5)).In addition,20 samples of healthy single pregnant women of childbearing age were randomly collected in early,middle and late pregnancy to verify the established reference interval.When no more than two PRL measurements in each group exceeded the established reference interval,they were considered validated.Results A total of 170 participants were included in the early pregnancy group,229 participants in the middle pregnancy group and 130 participants in the late pregnancy group.There were significant differences in serum PRL levels in pregnant women at different stages of pregnancy.With the increase of gestational age,serum PRL level increased.The reference intervals of serum PRL in early,middle and late pregnancy were 477-4270 mIU/L,1060-6574 mIU/L and 3497-18274 mIU/L,respectively.All the established reference intervals were verified.Conclusion This study has established the reference interval of serum PRL during pregnancy of healthy single pregnant women of childbear⁃ing age in Suzhou area,to provide help for clinical rational application of this index,and further reference for the prevention of pregnancy⁃related diseases.

关 键 词：泌乳素 孕妇 参考区间 妊娠期 

分 类 号：R714[医药卫生—妇产科学] R446.1[医药卫生—临床医学]