Hoffmann法建立北京地区成人载脂蛋白A1和载脂蛋白B的参考区间  

Establishment of reference interval of apolipoprotein A1 and apolipoprotein B for adults in Beijing by Hoffmann method

作　　者：杨郝亮 张萍[2] 武利涛[1] 郝金莉 丁然[1] 陈维华 刘爱禹 周航宇 Yang Haoliang;Zhang Ping;Wu Litao;Hao Jinli;Ding Ran;Chen Weihua;Liu Aiyu;Zhou Hangyu(Department of Clinical Laboratory Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院检验科,100700 [2]北京市通州区疾病预防控制中心实验室

出　　处：《北京医学》2025年第1期69-72,共4页Beijing Medical Journal

基　　金：2023年度慢性病防治与健康教育科研项目(BJMB0012023024015)。

摘　　要：目的采用Hoffmann法建立北京地区成人载脂蛋白A1(apolipoprotein A1,ApoA1)和载脂蛋白B(apolipoprotein B,ApoB)的参考区间。方法选取2021年1月至2023年12月北京中医药大学东直门医院健康管理中心进行体检的表观健康人群940名,收集研究对象的ApoA1和ApoB数据。采用Kolmogorov-Smirnov法检验数据的正态性,使用Box-Cox法转换非正态数据,使其满足正态分布;应用Hoffmann法建立ApoA1和ApoB的参考区间,分别使用参考变化值(reference change value,RCV)和厂商提供的参考区间验证ApoA1和ApoB参考区间。结果940名研究对象中男621名、女319名;年龄21~86岁,平均(42.6±13.6)岁。男性和女性ApoA1的参考区间分别为0.96~1.61 g/L和1.06~1.84 g/L,男性和女性ApoB的参考区间分别为0.63~1.04 g/L和0.58~0.98 g/L。不同性别和不同年龄ApoA1和ApoB参考区间比较的分析结果显示,男性和女性ApoA1和ApoB参考区间的差异均有统计学意义(P<0.05),<60岁和≥60岁参考区间的差异无统计学意义(P>0.05)。与厂商提供的参考区间相比,不同性别和不同年龄ApoA1和ApoB参考区间的上限相对偏差(d上)和下限相对偏差(d下)均小于对应的RCV(20.09%和21.42%)。结论北京地区成人ApoA1和ApoB的参考区间存在性别差异。建议应针对男性和女性设立不同的ApoA1和ApoB参考区间。Objective To establish the reference interval of apolipoprotein A1(ApoA1)and apolipoprotein(ApoB)for adults in Beijing by Hoffmann method.Methods A total of 940 apparently healthy people for physical examination in the Health Management Center of Dongzhimen Hospital of Beijing University of Chinese Medicine from January 2021 to December 2023 were selected,and the data of ApoA1 and ApoB were collected.Kolmogorov-Smirnov method was used to test the normality of the data,and non-normal data were transformed by Box-Cox to meet the normal distribution.The reference intervals of ApoA1 and ApoB were established by Hoffmann method,and the reference intervals of ApoA1 and ApoB were verified by reference change value(RCV)and reference intervals provided by manufacturers respectively.Results There were 621 males and 319 females among the 940 subjects,and the age ranged from 21 to 86 years,with an average age of(42.6±13.6)years old.The reference intervals of ApoA1 for males and females were 0.96-1.61 g/L and 1.06-1.84 g/L,respectively,and the reference intervals of ApoB for males and females were 0.63-1.04 g/L and 0.58-0.98 g/L,respectively.The subgroups analysis by gender and age showed that there were statistically significant differences between males and females for both ApoA1 and ApoB(P<0.05),but no statistically significant difference between<60 years old and≥60 years old populations(P>0.05).Compared with the reference intervals provided by manufacturers,the upper limit relative deviation d upper and lower limit relative deviation d lower of the self-built reference intervals for ApoA1 and ApoB in all subgroups were all less than the corresponding RCV(20.09% and 21.42%).Conclusions There are gender differences in the reference intervals of adults ApoA1 and ApoB in Beijing.It is suggested that different reference intervals of ApoA1 and ApoB should be set for men and women.

关 键 词：Hoffmann法 参考区间 载脂蛋白A1 载脂蛋白B 

分 类 号：R446.1[医药卫生—诊断学]