2022—2023年我国GII.3[P12]诺如病毒暴发分子流行病学特征  

Molecular epidemiological characteristics of GII.3[P12]norovirus outbreaks in China,2022-2023

作　　者：曹丽娇 杨艳辉 杨舒婷 章青[1] 孔翔羽[1] 王宏[1] 靳淼[1] 段招军[1] Cao Lijiao;Yang Yanhui;Yang Shuting;Zhang Qing;Kong Xiangyu;Wang Hong;Jin Miao;Duan Zhaojun(National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,NHC Key Laboratory for Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,传染病溯源预警与智能决策全国重点实验室,卫生部医学病毒和病毒病重点实验室,北京102206

出　　处：《中华实验和临床病毒学杂志》2025年第1期27-33,共7页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解2022—2023年我国诺如病毒急性胃肠炎暴发及聚集性疫情GII.3[P12]重组株的分子进化特征。方法收集2022年至2023年诺如病毒暴发及聚集性疫情流行病学信息、病例信息、临床样本以及检测和基因分型信息;对GII.3[P12]重组株阳性样本进行核酸提取、全基因组扩增及序列分析;利用同源模拟方法,构建三维结构并预测抗原表位。结果2022年1月至2023年12月,我国诺如病毒暴发监测网络共报告1136起诺如病毒暴发及聚集性疫情,942起疫情成功获得基因型分型结果,以GII为主,占76.0%(716/942),GI和混合基因型占比分别为15.8%(149/942)和8.2%(77/942)。GII引起的诺如病毒疫情以GII.3[P12]为主(22.5%,161/716),其他主要基因型包括GII.17[P17](18.7%,134/716)、GII.4_Sydney 2012[P16](11.6%,83/716)和GII.6[P7](11.3%,81/716)。2022—2023年GII.3[P12]引起的疫情集中发生在2~3月份(54.0%,87/161),主要暴发场所为托幼机构和小学(87.5%),传播途径以人传人为主(68.9%),3~7岁儿童为主要易感人群(93.3%)。本研究获得25株GII.3[P12]重组株基因组序列,根据系统进化分析表明,我国2022—2023年GII.3[P12]重组株属于Cluster IV簇的sublineage b(7株)和sublineage c(18株)。通过表位预测分析,共预测得到11个线性表位和8个构象性表位,预测得到的线性表位与构象性表位存在重合位置,每一个构象性表位都是预测的线性表位的一部分,其潜在的抗原结合位点与受体结合位点均保守。结论GII.3[P12]重组株是2022—2023年引起我国诺如病毒暴发及聚集性疫情的流行株之一,其基因组未发生明显变异。ObjectiveTo understand the molecular evolutionary characteristics of the recombinant strain GII.3[P12]of norovirus acute gastroenteritis outbreaks and aggregated outbreaks in China from 2022 to 2023.MethodsEpidemiological information,case information,clinical samples,as well as detection and genotyping information of norovirus outbreaks and aggregated outbreaks from 2022 to 2023 were collected;positive samples of the GII.3[P12]recombinant strain were subjected to nucleic acid extraction,whole-genome amplification,and sequence analysis;and homology simulation method were used to construct a three-dimensional structure and predict antigenic epitopes.ResultsFrom January 2022 to December 2023,a total of 1136 norovirus outbreaks and aggregated outbreaks were reported in China′s norovirus outbreak surveillance network,and genotyping result were successfully obtained for 942 outbreaks,with GII dominating,accounting for 76.0%(716/942),and the proportion of GI and mixed genotypes being 15.8%(149/942)and 8.2%(77/942).Norovirus outbreaks caused by GII were dominated by GII.3[P12](22.5%,161/716),while other major genotypes included GII.17[P17](18.7%,134/716),GII.4_Sydney 2012[P16](11.6%,83/716)and GII.6[P7](11.3%,81/716).2022-2023 Outbreaks caused by GII.3[P12]were concentrated in February-March(54.0%,87/161),with the main outbreaks occurring in nursery and primary schools(87.5%),the mode of transmission was mainly human-to-human(68.9%),and the main susceptible population was children aged 3-7 years(93.3%).In this study,the genome sequences of 25 GII.3[P12]recombinant strains were obtained,and according to the phylogenetic analysis,it was shown that the GII.3[P12]recombinant strains in China in 2022-2023 belonged to the Cluster IV cluster of sublineage b(7 strains)and sublineage c(18 strains).A total of 11 linear and 8 conformational epitopes were predicted by epitope prediction analysis,and the predicted linear and conformational epitopes had overlapping positions,and each conformational epitope was part of the predicted

关 键 词：诺如病毒 基因型 分子进化 表位预测 

分 类 号：S85[农业科学—兽医学]