2022年山东省鼠类汉坦病毒携带情况调查及其全基因组序列分析  

Investigation of hantavirus carriage in rodents and whole-genome sequence analysis in Shandong province,2022

作　　者：刘昱薇 姚明晓 张金艳 段青 逄博[2] 翟文济[2] 李仁鹏 寇增强[2] Liu Yuwei;Yao Mingxiao;Zhang Jinyan;Duan Qing;Pang Bo;Zhai Wenji;Li Renpeng;Kou Zengqiang(School of Public Health,Shandong Second Medical University,Weifang 261053,China;Institute of Infectious Disease Prevention and Control,Shandong Center for Disease Control and Prevention,Jinan 250014,China;Linyi City People’s Hospital,Linyi 276002,China;Shandong Center for Health Science and Talent Development,Jinan 250014,China)

机构地区：[1]山东第二医科大学公共卫生学院,潍坊261053 [2]山东省疾病预防控制中心传染病防制所,济南250014 [3]临沂市人民医院,临沂276002 [4]山东省卫生科技与人才发展中心,济南250014

出　　处：《中华实验和临床病毒学杂志》2025年第1期56-61,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：山东省医药卫生科技发展计划(202112050731);山东省疾病预防控制中心青年创新基金项目(QC-202302)。

摘　　要：目的分析2022年山东省淄博市鼠类携带汉坦病毒情况及病毒的遗传进化特征,为肾综合征出血热的科学防控提供参考依据。方法采用实时荧光定量RT-PCR检测试剂盒(Real-Time PCR Method)检测鼠肺中汉坦病毒核酸并鉴定汉坦病毒种类。设计引物,分节段扩增各基因片段,采用二代测序法对PCR扩增产物进行汉坦病毒全基因组测序。序列拼接采用DNA Star的子软件Seq Man 7.1.0.44进行,应用MEGA 7.0和Bioedit软件进行序列比对和进化分析。结果本次调查共捕获宿主动物270只,经检测共13份鼠肺样本为汉坦病毒阳性,带毒率为4.8%。成功获得4株汉坦病毒全基因组序列,均为汉城(SEOV)型。4份汉坦病毒阳性样本M基因核苷酸同源性较高,属于SEOV的S3亚型分离株,与河北、辽宁和北京的毒株具有高度相似性。核蛋白和糖蛋白免疫表位氨基酸序列与疫苗株Z37一致。结论本研究成功测定了山东省淄博市4株汉坦病毒的全基因组序列,其基因型以SEOV型为主,亚型为S3亚型,同亚型病毒基因同源性高,无明显变异。关键蛋白的免疫表位比对表明目前疫苗可能对当地流行株具有保护潜力,但仍需要进一步深入研究。ObjectiveTo analyze the situation of rodents carrying Hantavirus and the genetic and evolutionary characteristics of the virus in Zibo city,Shandong province in 2022,and provide reference for the scientific prevention and control of hemorrhagic fever with renal syndrome(HFRS).MethodsReal-time quantitative PCR(RT-qPCR)was used to detect hantavirus(HV)nucleic acids in rodent lung tissues and identify HV genotypes.Each nucleic acid fragment was designed to amplify various gene fragments by segment,and the whole genome of Hantavirus was sequenced by second generation sequencing.Sequence assembly was performed using SeqMan 7.1.0.44,a subprogram of DNAStar.Sequence alignment and evolutionary analysis were conducted using MEGA 7.0 and BioEdit software.ResultsA total of 270 host animals were captured in this survey.Among them,13 rodent lung samples tested positive for Hantavirus,resulting in a virus-positive rate of 4.8%.The full-genome sequences of four hantavirus strains were successfully obtained,all identified as Seoul virus(SEOV)genotype.Four Hantavirus-positive samples showed high nucleotide sequence homology in the M gene and belonged to the SEOV S3 subtype.These strains exhibited high similarity with those from Hebei,Liaoning,and Beijing.The amino acid sequences of the nucleoprotein and glycoprotein immunogenic epitopes were identical to those of the vaccine strain Z37.ConclusionsThis study successfully determined the full genome sequences of four hantavirus strains from Zibo city,Shandong province.The genotypes are primarily SEOV,with the subtype being S3.The homology of genes within the same subtype is high,with no significant variations observed.The alignment of immune epitopes in key proteins suggests that the current vaccine may provide protection against locally circulating strains,but further in-depth research is still required.

关 键 词：汉坦病毒 鼠类 全基因组 

分 类 号：R37[医药卫生—病原生物学]