2020年我国D3亚型乙肝病毒的基因组特征和进化分析  

Genomic characterization and evolutionary analysis of hepatitis B virus subgenotype D3 in China in 2020

作　　者：向晖 张爽[1] 王锋[1] 邱丰[1] 王富珍[2] 沈立萍[1] 苏秋东[1] Xiang Hui;Zhang Shuang;Wang Feng;Qiu Feng;Wang Fuzhen;Shen Liping;Su Qiudong(NHC Key Laboratory of Medical Viruses and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Department of National Immunization Program,Chinese Center for Disease Control and Prevention,Beijing 100050,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,国家卫生健康委医学病毒和病毒病重点实验室,北京102206 [2]中国疾病预防控制中心免疫规划中心,北京100050

出　　处：《中华实验和临床病毒学杂志》2025年第1期62-68,共7页Chinese Journal of Experimental and Clinical Virology

基　　金：国家重点研发计划(2023YFC2306900)。

摘　　要：目的分析2020年我国乙型肝炎病毒(hepatitis B virus,HBV)D3亚基因型的基因组特征及进化起源。方法收集HBV D3亚型感染者的血清样本和基本信息,采用巢式PCR法扩增和测序技术获得HBV全基因序列,利用生物分析软件进行系统发育、核苷酸同源性、S基因氨基酸突变和进化速率分析。结果获得14份D3亚型HBV样本的全基因序列。与97条参考序列对比发现和样本序列同源性最高的序列来自印度、伊朗、蒙古及中国,同源性在96.0%-97.9%之间。14例D3亚型株中发现了24种氨基酸突变,其中与免疫逃逸相关的突变包括T131A、Y134F和T140I。HBV D3亚型的遗传多样性水平在1975年以前缓慢上升,在1975—2000年间相对稳定,2000年之后呈下降趋势。进化速率分析显示样本QGLD D3-02、03与伊朗的参考株在1872年起源于共同祖先,其他12例样本QGLD D3-04~17与蒙古的参考株在1815年起源于共同祖先。结论我国HBV D3亚型基因序列与印度、伊朗、蒙古和中国的参考序列同源性最高,进化速率分析揭示了14例D3亚型HBV与蒙古、伊朗的参考株起源于共同祖先,研究结果丰富了我国HBV D3亚型的序列和进化信息,为HBV分子流行病学研究提供参考依据。ObjectiveTo analyze the genetic characteristics and evolutionary origin of hepatitis B virus(HBV)subgenotype D3 in China in 2020.MethodsSerum samples and demographic details from patients infected with HBV D3 subgenotype were collected.HBV genomic sequences were obtained by nested PCR amplification and subsequent sequencing.Phylogenetic analysis,nucleotide homology,amino acid mutation and evolution rate of the S protein were conducted by comparing with reference sequence using bioinformatics tools.ResultsThe complete HBV gene sequences of 14 samples of D3 subtype HBV were obtained.Compared with 97 reference sequences,it was found that the sequences with the highest homology were from India,Mongolia,Iran and China,with the homology ranging from 96.0%to 97.9%.Mutations of 24 amino acids were found in 14 strains of D3 subtype.Among them,T131A,Y134F and T140I were associated with immune escape-related mutations.The genetic diversity of HBV D3 subtype increased slowly before 1975,remained relatively constant from 1975 to 2000,and began to decline after 2000.Evolutionary rate analysis showed that samples QGLD D3-02 and 03 originated from a common ancestor with the Iranian reference strain in 1872,and the other 12 samples QGLD D3-04-17 originated from a common ancestor with the Mongolian reference strain in 1843.ConclusionsThe gene sequence of HBV D3 subtype in China had the highest homology with reference sequences from India,Iran,Mongolia and China.Evolutionary rate analysis revealed that 14 cases of HBV D3 subtype originated from a common ancestor with reference strains from Mongolia and Iran,which enriched the sequence and evolution information of HBV D3 subtype and provided a reference basis for the molecular epidemiological study of HBV.

关 键 词：乙型肝炎病毒 D3基因亚型 分子流行病学 氨基酸突变 进化速率 

分 类 号：R51[医药卫生—内科学]