Resveratrol inhibit the proliferation and migration of HepG2 cells through SIRT1-MAPK signaling pathway  

作　　者：Chen-Chen Feng Yi-Jiao Xu Yu Chen Wan-Wei Yang Xiao Wei Ren-Dong Zheng 

机构地区：[1]Department of Quality Management,Jiangsu Province Blood Center,Nanjing 210042,China [2]Department of endocrinology,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China [3]Department of Clinical Laboratory,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China.

出　　处：《Food and Health》2025年第1期22-28,共7页食品和健康(英文)

基　　金：supported by Natural Science Foundation of Nanjing University of Chinese Medicine(XZR2023010);Traditional Chinese Medicine Science and Technology Project of Jiangsu Province(QN202212);Guidance Program of Jiangsu Commission of Health(Z2020046).

摘　　要：Background: Resveratrol is a widely recognized anti-inflammatory and antioxidant agent,and it has been suggested to possess anti-tumor effects. But the effect of resveratrol onhepatocellular carcinoma and its molecular mechanisms are unknown. This study confirmedthe effects of resveratrol on HepG2 cell proliferation and migration, and the underlyingmechanism. Methods: Viability of resveratrol (0-200 μmol/L)-treated HepG2 cells wasdetected by CCK-8. Wound healing assay was employed to evaluate cell migration. Theexpression levels of proteins including Bcl-2, Bax, Caspase3, SIRT1, and components of theMAPK pathway were analyzed via Western blot. Results: Resveratrol significantly inhibitedthe migration and proliferation of HepG2 cells at concentrations above 100 μmol/L(P<0.01). The expression of Bax, cleaved Caspase3 and SIRT1 was up-regulate (P<0.05)and Bcl-2, p-JNK、p-p38 MAPK was down-regulate (P<0.05) by resveratrol. Conclusion:Resveratrol suppresses the proliferation and migration of HepG2 cells by activating theSIRT1 signaling pathway and inhibiting the JNK and p38 MAPK pathways.

关 键 词：RESVERATROL Hepatocellular carcinoma HepG2 cells SIRT1 MAPK 

分 类 号：R73[医药卫生—肿瘤]