湖北省某鸽场大肠埃希氏菌的分离鉴定与耐药性分析  

Isolation,Identification and Drug Resistance Analysis of Escherichia coli from A Pigeon Farm in Hubei Province

作　　者：王娟[1,2] 卢琴[2] 张记高[2] 程伊洛 张腾飞 刘静波[1] WANG Juan;LU Qin;ZHANG Ji-gao;CHENG Yi-luo;ZHANG Teng-fei;LIU Jing-bo(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010 China;The Key Laboratory of the Prevention and Control of Animal Bacterial Diseases of the Ministry of Agriculture and Rural Affairs,Hubei Provincial Key Laboratory of Livestock and Poultry Pathogenic Microbiology,the Institute of Animal Husbandry and Veterinary Medicine of Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]农业农村部畜禽细菌病防治制剂创制重点实验室,畜禽病原微生物学湖北省重点实验室,湖北省农业科学院畜牧兽医研究所,湖北武汉430064

出　　处：《动物医学进展》2025年第5期134-138,共5页Progress In Veterinary Medicine

基　　金：“十四五”国家重点研发计划项目(2022YFD1800400);湖北省自然科学基金重点类项目(2021CFA019)。

摘　　要：大肠杆菌病是危害鸽养殖业的重要细菌性传染病,然而相关的流行及耐药数据却相对较少。为研究湖北省鸽场大肠埃希氏菌的流行及耐药情况,采集了肉鸽屠宰后的肝、脾、肠内容物等285份,经伊红美蓝琼脂培养基分离培养,通过PCR鉴定,分离到了199株鸽源大肠埃希氏菌,分离率为69.82%。经过双重PCR扩增对菌株进行系统进化群鉴定,筛选出高致病群菌株60株(B2群34株,D群26株),占分离株的30.15%。采用药敏纸片法对60株高致病群菌株进行了12种抗菌药物的耐药性分析,结果表明,这些菌株对林可霉素、氟苯尼考、四环素、氨苄西林、红霉素和甲氧苄氨嘧啶的耐药率高于95%,而对大观霉素、替加环素、头孢噻肟、环丙沙星、多黏菌素B和美罗培南等较为敏感。所有菌株均耐3种以上的抗菌药物,最多可耐9种抗菌药物,其中51.67%的菌株耐6种抗菌药物,占比最多。研究结果可为湖北省鸽场大肠杆菌病的预防与合理用药提供科学数据。Colibacillosis is an important bacterial infectious disease affecting pigeon breeding industry.However,t here are relatively few data on the prevalence state and drug resistance of Escherichia coli.In order to study the prevalence state and drug resistance of Escherichia coli in pigeon farms in Hubei province,285 samples of liver,spleen and intestinal content were collected after slaughter of meat pigeons.The pathogens in samples were isolated by eosin methylene blue agar medium and identified by PCR.199 strains of Escherichia coli were isolated from pigeons,with an isolation rate of 69.82%.The phylogenetic groups of the strains were identified by duplex PCR,and 60 strains were highly pathogenic(34 strains in group B2 and 26 strains in group D),accounting for 30.15%of the isolates.The drug resistances of 60 strains of high pathogenic group to 12 antimicrobials were analyzed by drug susceptibility disk method.The results showed that the resistance rate of these strains to lincomycin,florfenicol,tetracycline,ampicillin,erythromycin and trimethoprim was higher than 95%,but they were sensitive to spectinomycin,tigecycline,cefotaxime,ciprofloxacin,polymyxin B and meropenem.All the strains were resistant to more than 3 antimicrobials,and up to 9 antimicrobials,of which 51.67%were resistant to 6 antimicrobials,accounting for the largest proportion.This study can provide scientific data for the prevention and rational drug use of Escherichia coli in pigeon farms of Hubei province.

关 键 词：大肠埃希氏菌 分离鉴定 致病群 耐药性 鸽 

分 类 号：S852.612[农业科学—基础兽医学] S858.39[农业科学—兽医学]